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Application of seed storage protein marker for identification of some Groundnut (Arachis hypogaea L.) cultivars

Document Type : Research Paper

Author

Abstract

Peanut (Arachis hypogaea) is one of the most important edible oil grains of legume in the world, that understanding the genetic variation among its varieties in choosing the variation and transferring the useful genes is valuable.To study the genetic relationships and diversity of peanut varieties, 10 varieties of peanuts were planted in a research farm in Lasht-Nesha (Guilan) in a randomized complete block design with three replications in 2016. After harvesting the seeds peanut varieties, soluble protein was performed by the Bradford method and SDS-PAGE electrophoresis of seed proteins. In survey of the soluble protein, a local variety of Guilan (Goli) was in group A alone Separately, than the other varieties. Analysis of protein bands with Total lab software showed 17 bands visible on the vertical gel. The highest level of expression of protein was band 11 with a molecular weight of 26 kDa were observed in all genotypes. Cluster analysis of protein bands by Ward method and cut it in 50% similarity, divided genotypes in four groups. Genotypes 6, 7, 8, 9 and 10 in the first group, genotypes 3, 4 and 5 in the second group and genotypes 1 and 2 were separately in groups third and fourth. In general, the results showed that enough diversity in the seed proteins was observed among peanut varieties.

Keywords

References
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Applied Biology
Volume 31, Issue 2 - Serial Number 56
August 2018
Pages 42-51
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