Document Type : Research Paper

Author

Urmia UniversityAssistant Professor, Department of Environmental Science, Urmia Lake Research Institute, Urmia University, Urmia

Abstract

Phytoremediation is a low-cost and effective technology that removes pollutants, such as dye-containing effluents, from the environment through the use of plants. In the present study, the potential of Hydrocotyle vulgaris L. in biodegradation of malachite green dye was evaluated. The results revealed that the plant capability in decolorization of the dye is as high as 90%. Moreover, high ability of the plant in consecutive removal in long term repetitive experiments confirmed the dye biodegradation by the plant. Some biochemical and physiological responses of the plant treated with different concentrations of the contaminant were studied and the results showed that increasing in the dye concentration could lead to the notable increase in the activities of superoide dismutase and peoxidase enzymes, confirming important role of these two enzymes in plant adaptation to existence of malachite green in the environment. Additionally, it was found that enhance in plant biomass, temperature and pH could lead to the increase in decolorization efficiency. In contrast, increasing in the dye initial concentration led to the increase of C/C.

Keywords

 Ali, H. (2010) Biodegradation of synthetic dyes-A review. Water, Air & Soil Pollution 213: 251-273.
Ansari, A.A.,  Khan, F.A. (2008) Remediation of eutrophic water using Lemna minor in a controlled environment. African Journal of Aquatic Sciences 33: 275-278.
Aravindhan, R., Rao, J.R., Nair, B.U. (2007) Removal of basic yellow dye from aqueous solution by sorption on green alga Caulerpa scalpelliformis. Journal of Hazardous Materials 142,68-76.
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical  biochemistry72: 248-254.
Chance, B.  and Maehly, A.C. (1955) Assay of catalases and peroxidases. Pp. 764-775. in: S. P. Colwick and N. O. Kalplan (eds). Methods in Enzymolology, vol. 2, Academic Press. New York.
Chin, A. and Berube, P.R. (2005) Removal of disinfection by-product precursors with ozone-UV advanced oxidation process. Water Research 39, 2136-2144.
Dornates, A.R., Angelica, L., Zuniga, G. (2012) Phenoloxidases activity in root system and their importance in the phytoremediation of organic contaminants. Journal of Environmental Chemistry and Ecotoxicology 4: 35-40.
Epstein, E. (1972) Mineral Nutrition of Plants: Principles and perspectives. JohnWiley& Sons, Inc., 412 pp., New York.
Forgacs, E., Cserháti, T. and Oros, G. (2004) Removal of synthetic dyes from wastewaters: a review. Environment International 30: 953-971.
Halliwell, B. (2006) Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiology 141: 312-22.
Halliwell, B., Gutteridge, J.M.C. (2015) Free radicals in biology and medicine. 5th ed., Oxford University Press, 944 pp, Oxford, United Kingdom.
Hamed, B.H. and El-Khaiary, M.I. (2008) Kinetics equilibrium studies of malachite green adsorption on rice straw-derived char.  Journal of Hazardous Materials 153: 701-708.
Hartmut, K.L. (1987) Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Pp. 350-382. in: R.D. Lester Packer (eds). Methods in Enzymolology, vol. 148. Academic Press. London.
Husain, Q. (2010) Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: a review. Reviews in Environmental Sciences and Bio/Technology 9: 117-140.
Ionenko, I.F., Anisimov, A.V., Dautova, N.R. (2010) Effect of temperature on water transport through aquaporins. Biologia Plantarum 54, 488-494.
Irfan Dar, M., Ahmad Khan, F., Rehman, F. (2015) Responses of antioxidative defense system and composition of photosynthetic pigments in Brassica juncea L. upon imidacloprid treatments. Abiotic & Biotic Stress Journal 1: 3-15.
Jadhav, J.,  Phugare, S., Dhanve, R. and Jadhav, S. (2010) Rapid biodegradation and decolorization of Direct Orange 39 (Orange TGLL) by an isolated bacterium Pseudomonas aeruginosa strain BCH. Biodegradation 21: 453-463.
Jensen, R.D. and Taylor S. A. (1961) Effect of temperature on water transport through plants. Plant Physiology 36:  639-642.
Jha, P., Modi, N., Jobby, R., Desai, N. (2015) Differential expression of antioxidant enzymes during degradation of azo dye reactive black 8 in hairy roots of Physalis minima L. International Journal of Phytoremediation 17: 305-312.
Khataee, A.R., Dehghan, G., Ebadi, A., Zarei, M. and Pourhassan, M. (2010) Biological treatment of a dye solution by macroalgae Chara sp.: Effect of operational parameters, intermediates identification and artificial neural network modeling. Bioresource Technology 101: 2252-2258.
Khataee, A.R., Movafeghi, A., Torbati, S., Salehi Lisar, S.Y., Zarei, M. (2012) Phytoremediation potential of duckweed (Lemna minor L.) in degradation of C.I. Acid Blue 92: Artificial neural network modeling. Ecotoxicology and Environmental Safety 80, 291-298.
Khilji, S. and Bareen, F. (2008) Rhizofiltration of heavy metals from the tannery sludge by the anchored hydrophyte, Hydrocotyle umbellata L. African Journal of  Biotechnology 7: 3711-3717.
Liu, H., Weisman, D., Ye, Y.B., Cui, B., Huang, Y.H., Colon-Carmona, A., Wang, Z.H. (2009) An oxidative stress response to polycyclic aromatic hydrocarbon exposure is rapid and complex in Arabidopsis thaliana. Plant Science 176: 375-382. 
Obinger, C.,   Maj, M.,   Nicholls, P. and  Loewen, P. (1997) Activity, peroxide compound formation, and heme d synthesis in Escherichia coli HPII catalase. Archives of Biochemistry and Biophysics 342: 58-67.
Pan, X. and Zang, D. (2009) Removal of malachite green from water by Firmiana simplex wood fiber. Electronic Journal of Biotechnology 12: 1-10. 
Paterson, S.,   Mackay, D.,  Tam, D. and  Shiu, W.Y. (1990) Uptake of organic chemicals by plants: A review of processes, correlations and models. Chemosphere 21: 297-331.
Pilon-Smits, E. (2005) Phytoremediation. Annual Review of Plant Biology 56: 15-39.
Robinson, T.,   McMullan, G.,   Marchant, R. and Nigam, P. (2001) Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresource Technology77: 247-255.
Schmidt, M., Dehne, S., Feierabend, J. (2002) Post-transcriptional mechanisms control catalase synthesis during its light-induced turnover in rye leaves through the availability of the hemin cofactor and reversible changes of the translation effciency of mRNA. Plant Journal 31: 601-613.
Sooknah, R. and Wilkie, A. (2004) Nutrient removal by floating aquatic macrophytes cultured in anaerobically digested flushed dairy manure wastewater. Ecological Engineering 22: 27-42.
Srivastava, S., Sinha, R. and Roy, D. (2004) Toxicological effects of malachite green, Journal of Aquatic Toxicology 66: 319-329.
Streb, P., Michael-Knauf, A., Feierabend, J. (1993) Preferential photoinactivation of catalase and photoinhibition of photosystem II are common early symptoms under various osmotic and chemical stress conditions. Physiologia Plantarum 88: 590-598.
Torbati S. (2016) Artificial neural network modeling of biotreatment of malachite green by Spirodela polyrhiza: Study of plant physiological responses and the dye biodegradation pathway. Process Safety and Environmental Protection 99: 11-19.
Torbati, S., Khataee, A.R., Movafeghi, A. (2014) Application of watercress (Nasturtium officinale R. Br.) for biotreatment of a textile dye: Investigation of some physiological responses and effects of operational parameters. Chemical Engineerng Research and Design 92: 1934-41.
Vafaei, F., Movafeghi, A., Khataee, A.R., Zarei, M. and Salehi Lisar, S.Y. (2013a) Potential of Hydrocotyle vulgaris for phytoremediation of a textile dye: Inducing antioxidant response in roots and leaves. Ecotoxicology and Environmental Safety 93: 128-134.
Vafaei, F., Movafeghi, A., Khataee, A. (2013b) Evaluation of antioxidant enzymes activities and identification of intermediate products during phytoremediation of an anionic dye (C.I. Acid Blue 92) by pennywort (Hydrocotyle vulgaris). Journal of Environmental Sciences 25: 2214-2222.
Verma, A., Raghukumar, C., Verma, P., Shouche, Y. and Naik, C. (2010) Four marine-derived fungi for bioremediation of raw textile mill effluents. Biodegradation 21: 217-233.
Winterbourn, C.C., McGrath, B. M. and Carrell, R.W. (1976) Reactions involving superoxide and normal and unstable haemoglobins. Biochemistry Journal 155: 493-502.