Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis

Aazami, Somaye; Asgarani, Ezzat; Abdi Aali, Ahiya

doi:10.22051/jab.2011.3292

Document Type : Research Paper

Authors

¹ M. A. Department Biology of Alzahra University azami.

² Department Biology of Alzahra University

https://doi.org/10.22051/jab.2011.3292

Abstract

DNA extraction is the first stage of gene structure and function study, and finding an effective protocol for isolation DNA in genetic engineering and microbiology studies is essential. There are many different methods for DNA extraction which are interchangeable depending on the wall and membrane structure in the bacterium. Objective: The aim of this study was the assessment of different methods for DNA extraction from gram-negative bacterium (Pseudomonas aeruginosa) and the selection of effective protocol.Materials and methods: In this study three different methods of DNA extraction from gram-negative bacterium (Pseudomonas aeruginosa) is compared to determine the quantity and the quality of the DNA. These methods include lysis buffer containing proteinase K, DNG^TM-plus kit and boiling. In each method, ten samples were selected in a completely random plan and the concentration of DNA was measured using spectrophotometer, Nanodrop and agarose gel electrophoresis. Result: Variance analysis illustrated a meaning difference between three methods. Also average comparison using Duncan test illustrated meaning difference. Finally, boiling method was considered as the most suitable method with the highest extraction efficiency.

Keywords

References

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Applied Biology

Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis

References

References

Volume 23, Issue 2
January 2010
Pages 14-22

Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis Evaluation Of three methods of DNA extraction from Pseudomonas aeruginosa by Spectrophotometer nanodrop and gel electrophoresis

References

References

Volume 23, Issue 2January 2010Pages 14-22

Volume 23, Issue 2
January 2010
Pages 14-22