Document Type : Research Paper
Authors
Abstract
Bioleaching is the biological conversion of an insoluble metal compound to form soluble in water which was influenced by several parameters. One of the important microorganisms involved in the uranium bioleaching process, is Acidithiobacillus ferrooxidans. Based on the importance and the effects of environmental parameters in the uranium bioleaching process and bacterial activity, the aim of this study is to obtain the optimal values of 5 important parameters in the process of uranium bioleaching. For this purpose, the natives Acidithiobacillus ferrooxidans, APH medium and the ore from Anomali 2 of Saghand in Yazd used and different parameters affecting on the uranium extraction, including shaking, pH, temperature, pulp density, and initial ferrous iron concentration were studied at different levels. The results of this study indicated that the maximum yields of uranium extraction and Eh values were observed at 150rpm, pH= 2, temperature 35 ° C, PD=5% (w/v) and 10 g/L of [FeSO4.7H2O].
Keywords
- Abdollahy, M. Shojaosadati, S.A. Zare Tavakoli, H. Valivand, A. (2011)
Bioleaching of Low Grade Uranium Ore of Saghand Mine. Iranian Journal
of Chemistry and Chemical Engineering 30: 71-79.
Abhilash, S. Pandey, B.D. (2011) Role of ferric ions in bioleaching of uranium
from low tenor Indian ore. Canadian Metallurgical Quarterly 50: 102-112.
Akcil, A. Ciftci, H. Deveci, H. (2007) Role and contribution of pure and mixed
cultures of mesophiles in bioleaching of a pyretic chalcopyrite concentrate.
Mineral Engineering 20: 310-318.
Boon, M. Heijnen, J.J. (1998) Gas-liquid mass transfer phenomena in
biooxidation experiments of sulphide minerals. a review of literature data
Hydrometallurgy 48: 187-204.
Chen, D. Lin, J. Che, Y. Liu, X. Lin, J. (2011) Construction of recombinant
mercury resistant Acidithiobacillus caldus. Microbiological Research 166:
515-520.
Chen, P. Yan, L. Yue, X. Li, H. (2013) Optimal parameters for bioleaching
of realgar using Acidithiobacillus ferrooxidans under different growth
conditions and mathematical analysis. Biocatalysis and Biotransformation
31: 33-41.
Choi, M.S. Cho, K.S. Kim, D.S. Ryu, H.W. (2005) Bioleaching of uranium from
low grade black schists by Acidithiobacillus ferrooxidans. World Journal
of Microbiology & Biotechnology 21: 377-380.
Dong, Y. Lin, H. Wang, H. Mo, X. Fu, K. Wen, H. (2011) Effects of ultraviolet
irradiation on bacteria mutation and bioleaching of low-grade copper
tailings. Minerals Engineering 24: 870–875.
Golmohammadi, H. Rashidi, A. Safdari, S.J. (2012) Simple and rapid
spectrophotometric method for determination of uranium (vi) in low grade
uranium ores using arsenazo(III). Chemistry and Chemical Technology 6:
245-249.
Kock, S.H. Barnard, P. du Plessis, C.A. (2004) Oxygen and carbon dioxide kinetic
challenges for thermophilic mineral bioleaching processes. Biochemical
Society Transactions 32: 273-275.
Manaf, Z. Bageri, S.A. Oliazadeh, M. Watling, H. Yaghobi Moghaddam, M;
(2009) Column bioleaching of fne particle size fraction of Sarcheshmeh
low grade ore. Journal of Microbial Biotechnology 1: 41-47.
Munoz, J.A. Gonzalez, F. Ballester, A. Blazquez, M.L. (1993) Bioleaching of a
Spanish uranium ore. FEMS Microbiology Reviews 11:109-120.
Mustin, C. Berthelin, J. Marion, P. Donato, P. (1992) Corrosion and
electrochemical oxidation of a pyrite by Thiobacillus ferrooxidans. Applied
and Environmental Microbiology 58: 1175-1182.
Mousavi, S. Yaghmaei, S. Jafari, A. (2007) Influence of process variables
on biooxidation of ferrous sulfate by an indigenous Acidithiobacillus
ferrooxidans. Fuel 86: 993-999.
Olsen, G.J. Brierley. J.A. Brierly, C.L. (2003) Bioleaching review part B:
progress in bioleaching application of microbial processes by the minerals
industries. Applied Microbiology and Biotechnology 63: 249 – 257.
Rawlings, D.E. (2005) Characteristics and adaptability of iron- and sulfuroxidizing microorganisms used for the recovery of metals from minerals
and their concentrates. Microbiology Cell Factories 4: 13.
Ronald, M.A. (1997) Handbook of Microbiological Media, 2th edition, Robert
Stern, New York.
Rashidi, A. Roosta-Azad, R. Safdari, S.J. (2014) Optimization of operating
parameters and rate of uranium bioleaching from a low-grade ore. Journal
of Radioanalytical and Nuclear Chemistry 301: 341-350.
Rashidi, A. (2015) Uranium extraction from low grade ore using bioleaching
method. F87F053. Nuclear Science and Technology Research Institute.
Seifelnassr, A. Abouzeid, A-Z. (2013) Exploitation of bacterial activities in
mineral industry and environmental preservation: an overview Journal of
Mining 2013: 1-13.
Witne, J.Y. Philips C.V. (2001) Bioleaching of ok tedi copper concentratrni
oxygen and carbon dioxide-enriched air Minerals Engineering 14: 25-48
)