Document Type : Research Paper

Authors

• Mehdi Kakaei ¹
• Mohsen Mansouri ²

¹ Assistant Professor, Department of Agriculture (Plant Breeding and Genetics), Faculty of Sciences, Payame Noor University, PO BOX 19395-4697, Tehran-Iran.

² Persian medicine in combat and crisis research center.

Abstract

In this research was performed, cryopreservation to method vitrification with two types of solution protecting PVS2 and PVS3 in 7 times (20, 40, 60, 80, 100, 120 and 140 minutes). This experiment factorial was studied in the form of with completely randomized design (CRD) with two factors protective solution (two level) and time levels (seven level) and three replications. The results of analysis of variance for treatment time and soluble type in three traits, percentage germination, root and shoot length was significant at the level of 1% (P<0.01). Interaction between the time treatment in solution type for the length of root and shoot length were significant at the level of 1% (P<0.01) for germination percentage was insignificant at the level of 5% (P<0.05). Overall, analysis of variance showed that is effective the protective solution type and levels of treatment time in the process of vitrification and the cryopreservation Sesame plant in assessment indicators.

Keywords

• Oil
• Cryoprotectants
• Vitrification

Main Subjects

• Herbal