Document Type : Research Paper

Authors

1 Gonbad kavvos university

2 Faculty Member of Gonbad Kavoos University

Abstract

In order to micro-morphology study of seed Rubus L. seven seed specimens were collected from different habitats in North of Iran stereo and SEM microscopic methods were applied. The characters were evaluated included dimension, shape, size, trichome and ornaments on surface. The results demonstrated that the mean size of seed was 2-4 mm; the biggest pyrenes were seen in R.caesius, R.sanctus and smallest in R.hirtus, R. dolichocarpus. pyrenes were formed in different shapes e.g., reniform (R. caesius), triangular form (R.discolor), elliptical form (R.hirtus, R.dolichocarpus, R.hyrcanus), ovate form (R. persicus) and oblique form (R. sanctus). Ornaments on the endocarp surface were presented variably; proments of surface was varied from low to high in treated species. White color was seen in all examined species. Stellate and simple trichome just presented in R. sanctus. General results confirmed that pyrenes morphology of Rubus were valuable character for identification.

Keywords

Aalders, L.E. and Hall, I. (1966) A Cytotaxaonomic survey of the native blackberries of Nova Scotia. Canadian Journal of Genetics and Cytology. 8: 528-532.
Baas, J. (1936) Die Pflanzenwelt in denfunfaltesten Burgen von Zantoch. Zantoch, eine Burgim deutschen Osten. Leipzig.
Brochmann,C. (1992) Pollen and seed morphology of Nordic Draba (Brassicaceae): Phylogenetic and ecological implication. Nordic J B. 1: 657-673.
Bojnansky, V. and Fargasova, A. (2007) In: Atlas of seeds and fruits of central and East European flora: The Carpathian Mountains Region. Springer, Dordrecht. 1046 pp.
Dorofeev, P.I. (1963) The Tertiary Floras of Western Siberia, Moskva, Leningrad, p. 201-203.
Focke, W.O. (1910) Species Ruborum Monographiae generic. RubiProdromus. Bibliotheca Botanica 17: 1-120.
Focke, W.O. (1911) Species Ruborum.Monographic Genesis. RubiProdromus. Bibliotheca Botanica. 17: 121-223.
Focke, W.O. (1914) Species Ruborum.Monographic Genesis.RubiProdromus.Bibliotheca Botanica. 17: 1-274.
Gilli, A. (1969) Rubu  PP. 67- 75. In: Flora Iranica vol. 66 (ed. Rechinger, K. H.). Graz: Akademische Druck-u.-Verlagsanstalt.
Hedge, I.C. (1992) A global survey of the biogeography of the Labiatae, Advances in Labiatae Science: 7-17. Royal Bot. Gardens, Kew.
Hummer, K.E. (1996) Rubus diversity. Horticultural Science. 31: 182-183.
Klichowska, M. (1960) Znaleziska ros´linne ze Starego Kolobrzegu. Spraw. Arche-ologiczne. 11: 93–95.
Klichowska, M. (1972) Rosliny naczyniowez zbiorowiskach kulturowych Polski po´ "nocno-zachodniej. Pozn. Tow. Przyj. Nauk Prace Komisji Biologicznej. 35 (2): 3–61.
Kokawa, S. (1966) Late Cenozoic Floras of the Boso Peninsula, Japan. I. Upper Pleistocene floral change. Journal of Biology. Osaka City University. 17: 105–149.
Komarov, V.L. (1971) Flora of the U.S.S.R, Translated from Russian, Israel program for scientific translation jeursalem. 6: 6-45.
Lechnicki, F. (1956) Survey of vegetation and Fossil evidence of seed coat in Rubus in early middlaged. Warszawa-Krakow.. 110-111.
Lu, L.T. (1983) A study on the genus Rubus of china. Actaphyto taxonomic sinica. 21: 13-25.
Nybom, H. (1980) Germination in Swedish blackberries (Rubus L. subgen. Rubus). Botanical. Notiser 133: 619–631.
Reeve, R.M. (1954) Fruit histogenesis in Rubus strigosus. I. Outer epidermis, parenchyma and receptacle. American Journal Botanical. 41: 152–159.
Reeve, R.M. (1954b) Fruit histogenesis in Rubus strigosus. II. Endocarp tissues. American Journal Botanical. 41: 173–180.
Richards, A., J. Kirschner, J., Stepanek, J. and Marhold, K. (1996) Apomixis and taxonomy: an introduction. Folia Geobotanica phytotaxonomica 31: 281-282.
Rose, R.C. (1919) After-ripening and germination of seed of Tilia, Sambucus, and Rubus. Botanical Gazette. 67: 281-308.
Satomi, N. and Naruhashi, N. (1971) Seed of  Japanese Rubus. I. Morphology. Kanazawa Daigaku Rigakubu fuzoku Shokubutsuen Nenpo. 4: 1-17.
Sharifnia, F. and Behzadi Shakib, S. (2012) Epidermal petal patterns of 13 Iranian Rubus L. (Rosaceae) species. Annals of biological Research. 3 (6): 2734-2740.
Szafer, W. (1961) Miocen´ ska flora ze Starych Gliwic na Slasku (Tortonian mosses from Stare Gliwice in Silesia). Prace Instytut. Geologiczne. 11: 1–238.
Tomlik-Wyremblewska, A., Zielinski, J. and Guzicka, M. (2010) Morphology and anatomy of blackberry pyrenes (Rubus L., Rosaceae) Elementary studies of the European representative of the genus Rubus L. Flora 205: 370-375.
 Thompson, M.M. (1995) Chromosome number of Rubus species at the National Clonal Germplasm Repository. Horticultural Science. 30: 1447-1452.
Thompson, M. (1997) Survey of chromosom numbers in Rubus (Rosaceae: Rosoidese). Annals of the Missouri Botanical Garden. 84 (1): 128-164.
Wada, S. (2009) Evaluation of Rubus Seed Characteristics: Seed Coat Morphology, Anatomy, Germination Requirements and Dormancy Breaking, Ph. D. Dissertation, Horticulture. Oregon State University, Corvallis, p. 207.
Wada, S. and Reed, B.M. (2008) Morphological analysis of Rubus seed. Acta Horticultural. 782: 67–74.
Wada, S. and Reed, B.M. (2010) Seed coat morphology differentiates blackberry cultivars. Journal of American Pomology Society. 64: 151–160.
Wada, S. and Reed, B.M. (2011 a) Optimized scarification protocols improve germination of diverse Rubus germplasm. Scientia Horticulturae. 130, 660–664.
Wada, S. and Reed, B.M. (2011 b) Standardizing germination protocols for divers raspberry and blackberry species. Scientia Horticulturae. 132 , 42-49.