نوع مقاله: مقاله پژوهشی

نویسنده

. استادیار گروه میکروبیولوژی دانشگاه آزاد اسلامی واحد رشت

چکیده

22 سویه باکتری قلیادوست از خاک‌های نمکی قلیایی استان گیلان در محیط milk Agar به‌منظور بررسی توانایی تولید آنزیم آلکالین پروتئاز جداسازی شد. از میان باکتری‌های جداسازی شده، اعضای جنس باسیلوس بیشترین توانایی تولید را نشان دادند. بررسی اثر درجه‌ی حرارت، زمان انکوباسیون، منبع کربن، ازت و pH نشان داد که بهترین شرایط تولید، به‌ترتیب در C ْ40 به‌مدت 20 ساعت با استفاده از 5/1% گلوکز (وزنی به حجمی)، 1% سولفات آمونیوم (وزنی به حجمی) و 5/9 = pH است

کلیدواژه‌ها

عنوان مقاله [English]

Production and optimization of alkaline protease from Bacillus species isolated from soil

نویسنده [English]

  • Maasoumeh Anvari

Department of Microbiology, Islamic Azad University, branch of Rasht, Rasht, Iran

چکیده [English]

22 strain of alkalophilic bacteria isolated from Guilan alkali soils were screened on milk agar medium for their ability to produce alkaline protease. Among isolated bacteria, bacillus spp. was show the highest enzyme production ability. Maximum enzyme production was achieved with sugar 1.5% w/v (as sole carbon source), ammonium sulfate 1% (as sole nitrogen source), pH= 9.5 and 40˚C over a 24 hrs. incubation

کلیدواژه‌ها [English]

  • alkaline protease
  • Bacillus spp
  • alkalophilic

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