Herbal
Mahideh Habibi kaleibar; sarah khorasaninejad; Mostafa Ebadi Nahari; Ghasem Karimzadeh; mohsen farhadpour; Christian Gruber
Abstract
Introduction: The genus Viola is the largest genus of the Violaceae and includes 525-600 species, of which 19 species are native to Iran. Methods: For the first time, micromorphological studies of leaf characters such as shape, dimensions, and density of stomata and trichomes in 6 species of Violets ...
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Introduction: The genus Viola is the largest genus of the Violaceae and includes 525-600 species, of which 19 species are native to Iran. Methods: For the first time, micromorphological studies of leaf characters such as shape, dimensions, and density of stomata and trichomes in 6 species of Violets were carried out using scanning electron microscopy (SEM) to identify useful characters for the identification of species. The characteristics of the leaf such as the dimensions and density of stomata and trichome were measured by image J V.1.52 software. The information was obtained from leaf micromorphological properties were analyzed using SPSS V. 26. Cluster analysis was used by WARD method to determine the species affinity. Results and discussion: The results of the microscopic analysis of the species in terms of micromorphological properties, classified 7 types of trichomes and stomata into 4 groups. The results of the microscopic analysis showed that the trichomes in the studied species are conical, triangular, and uncinate in shape, and there are 3 states: lying, raised, and semi-raised. The longest trichome in V. hirta observed. In all species, stomata were seen as oval in shape. The largest stomatal size was related to V. occulta species and the highest stomatal density was also related to V. hirta species. The results showed that micromorphological characters of six species of viola species, especially V. hirta species identification and separation.
