Biotechnology
Davoud Sadeghi; Mostafa Bakhshi; Mir-Morteza Sadat Ebrahimi; Shahram Nazarian; Mehdi Zeinoddini
Abstract
Introduction: Ricin is a glycoprotein poison found in the castor plant, which consists of two chains A (RTA) and B (RTB). There are two protein vaccine candidates based on chain A including RiVax and RVEc, against poisoning caused by this toxin. Although various studies have been conducted on the immunogenicity ...
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Introduction: Ricin is a glycoprotein poison found in the castor plant, which consists of two chains A (RTA) and B (RTB). There are two protein vaccine candidates based on chain A including RiVax and RVEc, against poisoning caused by this toxin. Although various studies have been conducted on the immunogenicity of RiVax alone and with the help of adjuvant, modern controlled release systems such as nanoparticles have not been used to improve the immunogenicity of this protein. The aim of this study is to manufacture polylactic-co-glycolic acid (PLGA) nanoparticles containing RiVax with a slow release rate and to evaluate the physicochemical parameters and immunogenic potential of system compared to protein alone. Methods: First, after purifying RiVax, the water-oil-water emulsion method was used to load RiVax into poly lactic acid-co-glycolic acid (PLGA) nanoparticles. After investigating the physicochemical properties of NPs containing RiVax, they were administered to different groups of mice. Results: The results showed that due to the use of PLGA polymer with a higher ratio of polylactic acid than polyglycolic acid, the protein release pattern from the nanoparticle was slow (12% protein in 40 days) and the immunization results showed a difference in response. Safety of nanoparticles containing RiVax compared to RiVax alone was not demonstrated. Conclusion: it was determined that despite the various researches and positive results related to the nature of immunogenicity enhancement by PLGA nanoparticles, one of the important factors in the enhancement of immunogenicity of these nanoparticles is the ratio of lactic acid to glycolic acid
