Herbal
mehrdad salehzadeh; saeedeh dehghanpour farashah; mohammad bagher hassanpouraghdam
Abstract
Introduction: Cucumber mosaic virus is a dominant disease in cucumber and many other crops, which causes a lot of damage worldwide and in Iran. In recent years, the use of defense inducers has received much attention. Methods: In the present study, we tried to investigate the defensive effects of sodium ...
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Introduction: Cucumber mosaic virus is a dominant disease in cucumber and many other crops, which causes a lot of damage worldwide and in Iran. In recent years, the use of defense inducers has received much attention. Methods: In the present study, we tried to investigate the defensive effects of sodium nitroprusside as a biological stimulus on Cucurbita pepo which is considered susceptible to Cucumber mosaic virus. The plants were incubated at the full two-leaf stage by CMV with a concentration of 50 micrograms/ml. After 48 hours, CMV-infected plants were sprayed with SNP in three concentrations of 100, 200 and 300 micrograms/ml. Finally, the level of expression of coat proteins and virus replicase genes, the expression pattern of PAL, GPX1, LOX1, Pr1 and Pr3 genes as well as some morpho-physiological characteristics of Cucurbita plants were evaluated in three repetitions during three weeks. Results and discussion: The results showed that the plants treated with SNP showed an increase in the expression of the studied genes after the infection by CMV. So that, the treated plants showed an increase in the genes expression pattern depending on the concentration of SNP till the third week compared to the control plants. In addition, several physiological characteristics were also improved. In conclusion, the use of SNP can be considered as an effective methodology in the induction of systemic resistance in Cucurbita pepo plants against CMV.
