Herbal
Fatemeh Shakeri; Fatemeh Moradian; Pouyan Mehraban jobini; Mehran Rostami
Abstract
Subject: The present study was conducted with the aim of nanoemulsifying Thymus vulgaris essential oil to increase its stability and investigate its antibacterial effects on E. coli DH5α bacteria. Methods: First, the initial microemulsion was prepared in the oil phase and then it was transformed ...
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Subject: The present study was conducted with the aim of nanoemulsifying Thymus vulgaris essential oil to increase its stability and investigate its antibacterial effects on E. coli DH5α bacteria. Methods: First, the initial microemulsion was prepared in the oil phase and then it was transformed into a nanoemulsion by ultrasound. The amount of surface charge, viscosity, particle size and morphology of essential oil nanoemulsion was investigated by zeta potential device, DLS and TEM electron microscope. Results: The results showed that the zeta potential of nanoemulsion containing 10 and 30% essential oil was -11.5 and -11.1 mV, respectively. The morphology of the nanoparticles of both samples was spherical and the average size of the nanoemulsion nanoparticles with 10% and 30% essential oil was 43 and 30 nm, respectively. The results of the antibacterial effects of nanoemulsions showed that the nanoemulsion containing 10% essential oil (v/v), with a volume of 30 µl, and the nanoemulsion containing 30% essential oil (v/v), with a volume of 20 µl, had approximately 50% inhibitory effect (IC50) on the growth of E. coli DH5α. Also, the primary essential oil showed approximately 50% inhibition (IC50) on bacterial growth in 20 µl. Conclusion: According to the stability of the produced thyme essential oil nanoemulsions, it can be concluded that the antibacterial effects of this compound have increased compared to the original essence.
