biochemistry
Zohreh Noruzi Motlagh; Mahmood Akhavan Mahdavi; Reza Gheshlaghi
Abstract
Nile red is a fluorescent dye that is used to determine the amount of intracellular lipids. This method can detect very low amounts of intracellular lipid with high accuracy. But the intensity of Nile red fluorescence changes with time, and this problem makes it difficult to use it for a set of analyzes ...
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Nile red is a fluorescent dye that is used to determine the amount of intracellular lipids. This method can detect very low amounts of intracellular lipid with high accuracy. But the intensity of Nile red fluorescence changes with time, and this problem makes it difficult to use it for a set of analyzes that are performed in a time interval. Considering that it is not possible to use fresh Nile red solution for all analyses. So, methods should be used to maintain the fluorescence intensity of Nile red solution so that this reagent can be used reliably for lipid analysis. In this study, the fluorescence intensity of Nile red solution has been investigated according to the two factors of time and the number of times of melting and freezing. The results showed that the fluorescence intensity of Nile red solution decreases due to freezing. But if the frozen solution remains frozen until use and is only melted during use, it can be stored for a long time and the decrease in fluorescence intensity will be less than 10%. But if it is repeatedly thawed and frozen (4 times or more), the fluorescence intensity decreases to about 80%, which is no longer suitable for analysis. If the frozen reagent (once frozen) is used, for 0 to 4 μg/ml of lipid concentration (olive oil) from , 0.05 μg/ml of Nile red concentration is suitable, and the lipid is correctly identified and its concentration is determined.
