Mehdi Kakaei; Tayebeh Basaki
Abstract
Preservation of plant genetic resources is of particular importance. Using ultra-cold seed storage techniques, seeds can be stored for a long time at a much lower cost. In the present study, the cryopreservation of millet seeds by a verification method was investigated. The experiment in factorial format ...
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Preservation of plant genetic resources is of particular importance. Using ultra-cold seed storage techniques, seeds can be stored for a long time at a much lower cost. In the present study, the cryopreservation of millet seeds by a verification method was investigated. The experiment in factorial format (with two factors, the first factor in two levels of dewatering solution (PVS2 and PVS3) and the second factor in dewatering times in five- time levels (20, 40, 60, 80, and 100 minutes) and with a completely randomized design and it was done with three repetitions. The results of the analysis of variance showed that all traits (root length, stem length, and germination percentage) were significant in terms of treatment time at the level of 1% probability. Also, the type of solution was significant for root length and shoot length and the interaction effect of treatment time in solution type was significant only for shoot length at the level of 1% probability. Treatment of millet seeds with protective solutions for 60 minutes showed the highest germination rate compared to other treatments. The results showed that seeds treated with PVS3 solution had shorter stem length but longer root length than PVS2 solution. In general, cryopreservation can be considered as a suitable method to protect the germplasm of millet. PVS3 solution had the highest average germination percentage at 60 minutes, which is the best treatment composition for long-term storage of millet.
