Biotechnology
Taibeh Hadi Turan back; Fakhri Sadat Hosseini; fatemeh safakhah
Abstract
Introduction: Microalgae are organisms with diverse and valuable metabolites, and two microalgae, spirulina and chlorella, have received much attention due to their high protein content.Method: In this study, after the cultivation of spirulina and chlorella microalgae, they were extracted and their protein ...
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Introduction: Microalgae are organisms with diverse and valuable metabolites, and two microalgae, spirulina and chlorella, have received much attention due to their high protein content.Method: In this study, after the cultivation of spirulina and chlorella microalgae, they were extracted and their protein was extracted by sonicator and subjected to enzymatic hydrolysis with two enzymes, alkaline protease and pepsin, and using the test DPPH and their antioxidant properties were measured. Also, antiproliferative property with two cancer cell lines MCF-7 and Caco-2 and antimicrobial property with two bacteria Escherichia coli and Staphylococcus aureus, proteins of spirulina and chlorella microalgae were measured before and after enzymatic hydrolysis.Result: The survival rate of MCF-7 cancer cells after 48-hour treatment with a concentration of 200 µg/ml hydrolyzed protein of S.platensis and C.vulgaris was 21.60% and 37.65%, respectively, and this amount has decreased for Caco-2 cancer cells by 23.63% and 26.48%, respectively.. Also, the results showed that protein hydrolysis had a positive effect on improving antioxidant, antiproliferative and antimicrobial properties
