Biotechnology
Elahe Mirzaie Delbari; Jafar Vatandoost; Matin Jami Moeini; Eisa Kohan-Baghkheirati
Abstract
Introduction: Agrobacterium-mediated transformation is an efficient technology for induction of hairy roots in plants. In the present study, the effect of Agrobacterium rhizogenes strains and explant type on hairy root induction in alfalfa was investigated. Methods: A factorial experiment was conducted ...
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Introduction: Agrobacterium-mediated transformation is an efficient technology for induction of hairy roots in plants. In the present study, the effect of Agrobacterium rhizogenes strains and explant type on hairy root induction in alfalfa was investigated. Methods: A factorial experiment was conducted in a completely randomized design with three replications in Islamic Azad University, Sabzevar Branch. The studied factors were Agrobacterium rhizogenes strains at four levels (A13, 318, 15834 and A4) and explant type at two levels (leaf and hypocotyl). Results and discussion: The highest percentage of hairy root induction was obtained in leaf explants inoculated with bacterial strain A4, which was significantly higher than other strains of Agrobacterium rhizogenes. Inoculation with bacterial strain 318 caused the least induction of hairy roots in leaf explants and hypocotyl. Bacterial strains A13 and 15834 produced the highest number of hairy roots per leaf and hypocotyl explants, respectively. The lowest number of hairy roots in leaf explants was produced under inoculation with 318 strain. The highest and lowest hairy root length in leaf explants, were observed under inoculation with bacterial strains A4 and 318, respectively. In hypocotyl explants, there was no significant difference between strains of Agrobacterium rhizogenes in terms of number and length of hairy roots. The results of separation of PCR products using gel electrophoresis, confirmed the presence of rolB gene in the genome of hairy root cells. According to the results, inoculation of leaf explants with A4 strain of Agrobacterium rhizogenes is recommended to produce hairy roots in alfalfa.
