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Zahra Rashid Kalkhoran; MAHMOOD TALKHABI; Vahid Azizi
Abstract
Introduction: Breast cancer is the most common type of cancer among women in the world. Research is ongoing to find new and less risky ways to treat breast cancer. Recent studies on the effect of Alpha-ketoglutarate (AKG) on various cancers have shown its inhibitory effect on some cancers. Here, the ...
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Introduction: Breast cancer is the most common type of cancer among women in the world. Research is ongoing to find new and less risky ways to treat breast cancer. Recent studies on the effect of Alpha-ketoglutarate (AKG) on various cancers have shown its inhibitory effect on some cancers. Here, the effect of AKG on the proliferation and migration of 4T1 breast cancer cells was investigated. Methods: 4T1 cells were treated with different concentrations of AKG, the cell viability was calculated using the MTT test, and 160 μM of AKG was selected. 4T1 cells were cultured in the presence and absence 160 μM AKG, and the growth of 4T1 cells, population doubling time, colony forming potential, cell cycle profile and cell migration rate were investigated. Result and discussion: The results showed that AKG reduces the growth of 4T1 cells and increases the population doubling time in the treatment group compared to the control group. Also, AKG decreased the colony forming potential of 4T1 cells (in terms of colony number and size). AKG caused changes in the cell cycle profile in the treatment group, and the results of the scratch repair test also showed the effects of AKG in reducing the ability of cell migration in the treatment group compared to the control. The results of this study showed that AKG can reduce the survival and proliferation of breast cancer cells. It seems that the use of AKG along with other available treatments can help to control and treat breast cancer.
