Herbal
Sara Ramezani; Sayeh Jafari Marandi; Sedighe Arbabian; Fahimeh Salimpour
Abstract
Introduction: Although all flowering plants share similar stages of development in their vegetative and reproductive organs, there is potential for variation in organ conditions due to gene expression. Understanding flower development stages helps comprehend genetic mechanisms for plant organogenesis ...
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Introduction: Although all flowering plants share similar stages of development in their vegetative and reproductive organs, there is potential for variation in organ conditions due to gene expression. Understanding flower development stages helps comprehend genetic mechanisms for plant organogenesis and morphogenesis. Polygonum bistorta L. is a medicinal plant from the Polygonaceae family. The aim of this study is to examine the anatomical structure of vegetative meristems and investigate several stages of flower development in this species. Hence, this particular species has been gathered from Hamadan Province. Methods:Carmen Zaji, methyl green, hematoxylin, and eosin stains have been used to examine the histology of the plant. An electron microscope photo of the pollen has also been prepared. The Results and Discussion: The investigation has shown that the vascular system of the root and stem in dicotyledons is similar, with collateral vessels in the stem and alternate and pantropical in the root. The vegetative meristem is prominent and domed, and the pollen is spherical and split (tricoplate). The ovule develops into a slightly protruding fruit with thick, lignin-walled cells containing the embryo, which cotyledons fill the bulk of the seed. Calcium oxalate was not found in the studied organs.
Elham Basiri; Sayeh Jafari Marandi; Sedighe Arbabian; Ahmad Majd; Mohammad Ali Malboobi
Abstract
Phosphate fixation by chemical fertilizers, in addition to being costly, carries environmental risks. Today, fertile phosphate biofertilizer 2 contains two types of phosphate-solubilizing bacteria, decomposes insoluble phosphorus compounds and thus absorbable for the plant. The aim of this study was ...
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Phosphate fixation by chemical fertilizers, in addition to being costly, carries environmental risks. Today, fertile phosphate biofertilizer 2 contains two types of phosphate-solubilizing bacteria, decomposes insoluble phosphorus compounds and thus absorbable for the plant. The aim of this study was to evaluate the effect of fertile biofertilizers 2 Pseudomounas putida (Strain P13) and Pantoea aggilomerans (Strain P5) (only once at the beginning of the growing season) and potassium phosphate in three concentrations (0, 1.2 mM, 5 mM) (Until the end of the growing season,once a week with irrigation) on some developmental characteristics of Arabidopsis thaliana in greenhouse conditions, 15 hours of light, temperature 25 ° C, light intensity of 6000 lux was done in a randomized design. After cell-histology, slides were observed under a light microscope. The results showed a significant reduction in wood vascular density in the stems of plants treated with 5 mM and 1.2 mM potassium phosphate compared to the other two treatments. Also, 5 mM potassium phosphate increased the tunica layers and the special wall thickness of the tetrads. Cell proliferation of ovule primordium, pre-embryo, and suspensor was observed in plants treated with biofertilizer and 5 mM phosphate. Treatment with biofertilizer showed the highest average number of seeds produced. The application of fertile biofertilizer 2 in this experiment, with effects similar to potassium phosphate, increased the quantitative and qualitative yield of Arabidopsis. Biofertilizer can be effective as a safe alternative to increase soil productivity and plant growth in sustainable agriculture and minimize environmental pollution.
