Saeed Mollaei; Mahnaz Eslami; Mostafa Ebadi
Abstract
Despite a long history of medicinal use of Lepdium vesicarium, the phenolic compounds and biological activity studies have not been performed on different organs of the plant. So, after obtaining methanolic extract of the different organs (root, leave, stem, branch, and seed), the total phenolic and ...
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Despite a long history of medicinal use of Lepdium vesicarium, the phenolic compounds and biological activity studies have not been performed on different organs of the plant. So, after obtaining methanolic extract of the different organs (root, leave, stem, branch, and seed), the total phenolic and flavonoid contents were assayed by spectrophotometric methods, and antioxidant and cytotoxic properties were evaluated by DPPH and MTT methods, respectively, and then free and esterified phenolic acids were analyzed by HPLC. According to the results, the methanolic extracts of seed and leaf had the highest total phenolic content, and the maximum flavonoid content was related to the stem and seed extracts. The investigation of antioxidant and cytotoxic activity against MCF-7 cells showed that the highest antioxidant activity was related to the methanolic extract of seed, and the extracts of stem and seed had the highest cytotoxic activity. The results of phenolic acids analysis indicated that caffeic acid with the amounts of 1.44±0.16 and 1.11±0.09 mg/g dry weight was the main free phenolic acids in the leaf and branch extracts, respectively. Also, salycilic acid and m-coumaric acid with the amounts of 0.98±0.07 and 0.95±0.07 mg/g dry weight were the predominant esterified phenolic acid in the root and branch extracts, respectively. Therefore, based on the results, the seeds have the highest phenolic compounds, including caffeic, ferulic and gallic acids and due to its high antioxidant potential and cytotoxicity, it can be used in the pharmaceutical and food industries.
Samaneh Narimani; Mostafa Ebadi; Mohammad Pazhang; Saeid Mollaei
Abstract
L-asparaginase is an enzyme that catalyzes the hydrolysis of asparagine into aspartic acid and ammonia and is one of the most important enzymes in the pharmaceutical and food industries. The goal of this study was the isolation and identification of asparaginase producing endophytic fungi from Salvia ...
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L-asparaginase is an enzyme that catalyzes the hydrolysis of asparagine into aspartic acid and ammonia and is one of the most important enzymes in the pharmaceutical and food industries. The goal of this study was the isolation and identification of asparaginase producing endophytic fungi from Salvia nemorosa and the determination of their enzyme activities. In this study, different species of endophytic fungi were isolated from healthy leaves of S. nemorosa. A qualitative assay of asparaginase enzyme was performed on asparagine agar medium and its quantitative assay was performed on Czapexdox's medium by spectroscopic method with Nessler reagent. Morphological and molecular investigations indicated that the obtained isolates belonged to genera of Alternaria, Stemphylium Talaromyces, and Linnemannia . Different species were shown to be able to produce the asparaginase by creating a pink to a red color zone. Alternaria alternata was selected as the best enzyme-producing isolate by producing 2.34 U/mg asparaginase in Czapexdox's medium, and could be considered as a potential source for enzyme production.
