Herbal
mehrdad salehzadeh; saeedeh dehghanpour farashah; mohammad bagher hassanpouraghdam
Abstract
Introduction: Cucumber mosaic virus is a dominant disease in cucumber and many other crops, which causes a lot of damage worldwide and in Iran. In recent years, the use of defense inducers has received much attention. Methods: In the present study, we tried to investigate the defensive effects of sodium ...
Read More
Introduction: Cucumber mosaic virus is a dominant disease in cucumber and many other crops, which causes a lot of damage worldwide and in Iran. In recent years, the use of defense inducers has received much attention. Methods: In the present study, we tried to investigate the defensive effects of sodium nitroprusside as a biological stimulus on Cucurbita pepo which is considered susceptible to Cucumber mosaic virus. The plants were incubated at the full two-leaf stage by CMV with a concentration of 50 micrograms/ml. After 48 hours, CMV-infected plants were sprayed with SNP in three concentrations of 100, 200 and 300 micrograms/ml. Finally, the level of expression of coat proteins and virus replicase genes, the expression pattern of PAL, GPX1, LOX1, Pr1 and Pr3 genes as well as some morpho-physiological characteristics of Cucurbita plants were evaluated in three repetitions during three weeks. Results and discussion: The results showed that the plants treated with SNP showed an increase in the expression of the studied genes after the infection by CMV. So that, the treated plants showed an increase in the genes expression pattern depending on the concentration of SNP till the third week compared to the control plants. In addition, several physiological characteristics were also improved. In conclusion, the use of SNP can be considered as an effective methodology in the induction of systemic resistance in Cucurbita pepo plants against CMV.
Herbal
Eshagh shamsinow; Mohammad Ali Aazami; Mohamad Bagher Hasanpoor
Abstract
In this study evaluate the effects of grapes cultivar on tolerance of vitis vinifera grown in drought stress conditions. Plants were grown in pots and exposed to 0, 40, 60 and 80 percent of fild capacity drought stress with three different cultivars (perlet, khaliliand ghizil ouzum). The physiological ...
Read More
In this study evaluate the effects of grapes cultivar on tolerance of vitis vinifera grown in drought stress conditions. Plants were grown in pots and exposed to 0, 40, 60 and 80 percent of fild capacity drought stress with three different cultivars (perlet, khaliliand ghizil ouzum). The physiological parameters such as MDA, H2O,EC and morphological paramaters such as dry and fresh weight and chlorophyll fluorescence Fo,Fv, Fm, Fv/Fm, Y(II) Y(NO) the contents of chlorophyll a, chlorophyll b, and enzyme activities of (APX, GPX, and CAT) and prolin content and protein percent were determined. Grapes cultivars positively (ghizil ouzum) affected on growth and physiology statistically. The highest, proline content was significantly different under drought stress condition and ghizil ouzum treatments so that, enzyme activities of (APX, GPX, and CAT) are increased with enhancement of drought levels .interaction effects of drought and cultivars in CAT, APX and GPX activity were significantly different compared with control and increased with enhancement of stress levels so that most of the activity was in 60% fc concentration and ghizil ouzum cultivar. The amount of chlorophyll fluorescence parameters (Fm, and Fv, Fv/Fm, Yll, Y(N0) were significant changes in drought stress treatment and ghizil ouzum in 60% FC compared with control.
