Samaneh Narimani; Mostafa Ebadi; Mohammad Pazhang; Saeid Mollaei
Abstract
L-asparaginase is an enzyme that catalyzes the hydrolysis of asparagine into aspartic acid and ammonia and is one of the most important enzymes in the pharmaceutical and food industries. The goal of this study was the isolation and identification of asparaginase producing endophytic fungi from Salvia ...
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L-asparaginase is an enzyme that catalyzes the hydrolysis of asparagine into aspartic acid and ammonia and is one of the most important enzymes in the pharmaceutical and food industries. The goal of this study was the isolation and identification of asparaginase producing endophytic fungi from Salvia nemorosa and the determination of their enzyme activities. In this study, different species of endophytic fungi were isolated from healthy leaves of S. nemorosa. A qualitative assay of asparaginase enzyme was performed on asparagine agar medium and its quantitative assay was performed on Czapexdox's medium by spectroscopic method with Nessler reagent. Morphological and molecular investigations indicated that the obtained isolates belonged to genera of Alternaria, Stemphylium Talaromyces, and Linnemannia . Different species were shown to be able to produce the asparaginase by creating a pink to a red color zone. Alternaria alternata was selected as the best enzyme-producing isolate by producing 2.34 U/mg asparaginase in Czapexdox's medium, and could be considered as a potential source for enzyme production.
Saeed Mollaei; Mostafa Ebadi
Abstract
Bracket fungi are the most important macroscopic fungi that play a main role in the ecosystem. In this study, the morphological characters of Cerrena unicolor were evaluated and then, the effect of different solvents (n-Hexan, Dichloromethane, Ethyl acetate, and Methanol) on the extraction of phenolic ...
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Bracket fungi are the most important macroscopic fungi that play a main role in the ecosystem. In this study, the morphological characters of Cerrena unicolor were evaluated and then, the effect of different solvents (n-Hexan, Dichloromethane, Ethyl acetate, and Methanol) on the extraction of phenolic compounds, antioxidant properties, and cytotoxic activities of the extracts were investigated. Total phenol and flavonoid content, phenolic acid analysis, antioxidant properties, and cytotoxic activities on MCF-7 cells were evaluated by spectrophotometry, HPLC, DPPH, and MTT methods, respectively. The obtained results showed the basidiocarp was semicircular and wavy, and the upper surface was covered with small white to grayish brown hairs. Basidia were 25 µm in length, calvate and including 4 sterigmata with ellipsoid basidiospores. The results also indicated that the Methanolic extract had the highest total phenol and flavonoid contents compared with other extracts. Moreover,Benzoic acid, Protocatechuic acid, Gallic acid, and Cinnamic acid were the main phenolic acids of the Methanolic and Ethyl acetate extracts. In addition, the Methanolic extract showed the highest antioxidant activity in inhibiting DPPH radicals, while the Methanolic and Ethyl acetate extracts indicated the highest cytotoxic activity on MCF-7 cells. Therefore the Methanolic extract due to phenolic compounds could be proposed as a new sourceofnatural antioxidants with cytotoxic activity.
