Bahareh Zarei; Khadijeh Kiarostami; Monir Hosseinzade Namin; Mona Sorahinobar
Abstract
Silicon nanoparticles have distinctive physicochemical characteristics. They are able to enter into plants and impact the metabolisms of plants as well as improve plant growth and yield under unfavorable environmental conditions. This research was done in order to study the physiological effects of nanosilicon ...
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Silicon nanoparticles have distinctive physicochemical characteristics. They are able to enter into plants and impact the metabolisms of plants as well as improve plant growth and yield under unfavorable environmental conditions. This research was done in order to study the physiological effects of nanosilicon on Crocus sativus corm. The corms were treated with silicon nanoparticles in concentrations of 0, 9 and 18 mg L−1. The experiment was done as completely randomized design in three replicates. The results showed that nano-silicon treatment increased content of silicon, potassium, iron, zinc, magnesium and calcium contents in plants but did not affect the fresh and dry weight as well as protein content, significantly. Moreover, the number of daughter corms, malondialdehyde and proline content significantly increased in nano-silicon treated plants as compared to non-treated control. The maximum content of total phenolics and flavonoid content were observed in plants treated with 9 and 18 mgL-1 respectively. Based on the results, it can be concluded that the examined concentrations of silicon nanoparticles can increase saffron corm nutrient content, its capacity of antioxidant system by the production of secondary metabolites.
jamshid fooladi; nashmin fayazi hossini; khadijeh kiarostami
Abstract
Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytic acid The major storage form of phosphorous in plants. Monogastric animals, such as pig, poultry and fish are not able to metabolize phytic acid. Adding phytase to diet monogastric animals lead ...
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Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytic acid The major storage form of phosphorous in plants. Monogastric animals, such as pig, poultry and fish are not able to metabolize phytic acid. Adding phytase to diet monogastric animals lead to increased avalibility of phosphorus. The objective of the present study is to isolate and characterize phytase producing bacterial strains from rhizosphere of wheat. Phytase production was determined by production of clear zones around the colonies on the sodium phytate containing medium and the best phytase producing strain was identified using molecular analysis. Also, the isolate was tested for P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) containing tricalcium phosphate as the sole P source. Among the 11 bacterial isolates, forming clear peripheral zones on a turbid agar plate, Staphylococcus pasteuri isolated able high potential for phytase production.This study showed that , Staphylococcus pasteuri capable production phytase and phosphatase enzyme.
Zahra Bokaii; Khadigeh Kiarostami; Razieh Rahchamani
Abstract
Solanaceae, is one of the most important families of Angiosperm which has various edible and medicinal usages. Lycuim genus of this family contains different compounds such as carbohydrates and anti-oxidants. A medicinal species of this genus, L.barbarum, is proposed to have some polysaccharides with ...
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Solanaceae, is one of the most important families of Angiosperm which has various edible and medicinal usages. Lycuim genus of this family contains different compounds such as carbohydrates and anti-oxidants. A medicinal species of this genus, L.barbarum, is proposed to have some polysaccharides with anti-cancer properties. This study was carried out to detect and analyze the carbohydrate content of Lycium species grown in Iran and compare it with its medicinal sp., L.barbarum. Amount and quality of soluble sugars, reducing sugars and polysaccharide content of 6 species- L. ruthenicum, L. kopetdaghi, L. depr-essum sub sp. Turcomanicum and sub sp. angustifolium, L. edgworthii, L. shawii, L. makranicum were compared with each other and with L. barbarum. Amongst the studied species, L. markranicum had the highest amount of soluble leaf sugars, reducing sugars and polysaccharide content. The fruit polysaccharide content was highest in L. ruthenicum, and L. edgworthii had the highest soluble sugars and reducing sugar amounts. The studied species had close similarity in their leaf and fruit soluble sugar quality except L. shawii. No difference was seen in the quality of leaf polysaccharide content of the studied species. Fruit polysaccharide content of L. ruthenicum and L. kopetdaghi showed the most similarity with the L. barbarum. The results indicate that the Lycium species grown in Iran have the potential to produce various carbohydrates and are competitive to L. babarum.
Maryam Keshavarzi; Somaye Esfandani bozchaloyi; Khadijeh Kiarostami
Abstract
In present study whole plant extract was achieved by maceration for effective components, evaluation of antioxidant activity and total phenol and flavonoid for six Stellaria species and its close relatives comprising Myosoton aquaticum and Mesostemma kotschyanum (Caryophyllaceae, Alsinoides). Phenolic ...
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In present study whole plant extract was achieved by maceration for effective components, evaluation of antioxidant activity and total phenol and flavonoid for six Stellaria species and its close relatives comprising Myosoton aquaticum and Mesostemma kotschyanum (Caryophyllaceae, Alsinoides). Phenolic and flavonoid contents were measured by Foline-ciocalteu and Aluminum chloride methods. Results indicated that there are significant differences between average content of phenolic and flavonoid compounds and antioxidant activity in different species (P< 0.01). Observations showed that total phenolic components of eight studied species are between 1±0.028 to 8.18±0.69mgr. /gr. dry weight and total flavonoid contents between 1±0.026 to 6.7±0.59 mgr./gr. in dry weight. Most inhibitory activity of DPPH free radical was observed in S. alsinoides with IC50 equal to 3 ±0.1 mgr. /ml in dry weight
Firuze Alirezayi; Khadijah Kiarostami; Monia Hussein Zadeh Namin
Abstract
Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants ...
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Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants secondry methabolites with high antioxidant properties ,silver nitrate as abiotic elicitor used in tissue culture of Lavandula angutifolia. Concentrations of 1, 2, 4 and 6 mg/l of silver nitrate as abiotic elicitor added to the one-month calli from leaf explant grown in MS medium with , Naphthaleneacetic acid(NAA)2mg/l, Benzyl amino purine (BAP) 4mg/l.After a month calli were harvested and the analyses were performed.The results showed that with increasing concentration of silver nitrate in the culture medium, growth of calli was increased and increasing the amount of silver nitrate concentration up to 4mg / l, increased Rosmarinic acid, flavonoids ,phenolic compounds and antioxidant activity .But in higher concentration of 4 mg / l the toxic effects of silver ions revealed and reduced the amount of Rosmarinic acid,phenolic compounds and antioxidant activity.
Khadijeh Kiarostemi; Saeideh Sadri; Nasrin Abdamaleki; Ozra Saboura
Abstract
Salicylic acid (SA) is a naturally produced compound and has been implicated to play important roles in tolerance of plants against diverse biotic and abiotic stresses. Experiments were conducted to study the regulatory role of SA on antioxidant enzyme induction in two salt sensitive (Y3000) and relative ...
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Salicylic acid (SA) is a naturally produced compound and has been implicated to play important roles in tolerance of plants against diverse biotic and abiotic stresses. Experiments were conducted to study the regulatory role of SA on antioxidant enzyme induction in two salt sensitive (Y3000) and relative salt tolerant ( H420) cultivar of canola. The experiment was conducted in Hajiabad country, Qom. The seeds were cultivated as a completely randomized block. SA treatment carried out with 0, 0.5 and 1μM of SA solution in the early morning when the plants fourth leaf completely expanded. The samples were taken at up to120 (vegetative phase) days after germination for analysis. The results revealed that salicylic acid increased peroxidase, polyphnol oxidase and superoxide dismutase activities in Y300 cv. By contrast antioxidant enzymes activities decreased in H420 cv. PAGE gel electrophoresis analysis of antioxidant enzymes revealed that salicylic acid increased peroxidase activity and intensity in Y 300 cv. Poly phenol oxidase activity was unchanged and Cu,/ Zn-SOD was the most abundance isozyme. The activity of this izozyme increased in treated plants. Fe- SOD was observed in Y 300 cv. and Mn-SOD was observed in H420 cv. Therefore ROS involve in different signaling pathway in different compartments of the cell in salt sensitive and relative salt tolerant cultivars. As a result SA increased salt tolerance in salt sensitive cultivar by increasing antioxidant enzyme activity
Fatemeh Gholi Zadeh; Khadigeh Kia Rostami; Zahra Nazem Bokaee
Abstract
Damask rose is one of the most important medicinal and aromatic plants from economical point of view in Iran. Damask rose is commonly propagated by asexual methods. Conventional propagation of Rosa damascena is limited attributed to low adventitious root formation on cutting. In vitro culture has became ...
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Damask rose is one of the most important medicinal and aromatic plants from economical point of view in Iran. Damask rose is commonly propagated by asexual methods. Conventional propagation of Rosa damascena is limited attributed to low adventitious root formation on cutting. In vitro culture has became an alternative method for propagation of this plant species. The shoot single node segments included lateral buds were taken from bushes. Single-node explants were surface sterilized with sodium hypochlorite 1.2% for 15 min and cultured on MS and QL media supplemented with various concentrations of N6- benzyl adenine (BA) and kinetin (Kin). Shoot proliferation was superior on QL than in MS medium. The best growth regulator combination were 5mgl-1 BA and 1.5mgl-1 Kin.
