Mostafa Khoshhal Sarmast; hasan Salehi
Abstract
Potential influence of AgNPs on plant genetic transformation through Agrobacterium has not yet been addressed. Here we showed that the growth of Agrobacterium was suppressed in 10 µg/ml AgNPs but controlling the overgrowth of these bacteria would effectively necessitate a higher concentration of ...
Read More
Potential influence of AgNPs on plant genetic transformation through Agrobacterium has not yet been addressed. Here we showed that the growth of Agrobacterium was suppressed in 10 µg/ml AgNPs but controlling the overgrowth of these bacteria would effectively necessitate a higher concentration of AgNPs when tobacco explants have inoculated with A. rhizogenes. Research result indicated that applying more than 150 µg/ml AgNPs and more, resulted in leaf injury and application of 100 µg/ml of AgNPs was unable to suppress bacteria regrowth after co-cultivation with tobacco leaves. The concurrent application of the AgNPs and Cefotaxime with different concentration was investigated and results indicated that using 100 µg/ml of AgNPs along with 200 mg/l of cefotaxime lead to the lowest leaf injury and the highest regeneration potential. This application not only caused a reduction in heavy metal toxicity but also decreases excess concentrations of antibiotics during the course of transformation. TEM manifested that the AgNPs could suppress Agrobacterium growth by potentially anchoring to and penetrating the bacterial cell wall. Our results suggest that the simultaneous use of AgNPs along with Cefotaxime can suppress the overgrowth of Agrobacterium during plant transformation. The result of this experiment can open a new window for application of AgNPs with lower diameter in order to suppress bacteria overgrowth.
