shahla hashemi; Fereshteh Mohamadhasani
Abstract
Green synthesis by plant extract has achieved a growth in interest. In the present study, zinc oxide nanoparticles were synthesized by olive extract.Morphological and structural properties of the synthesized zinc oxide nanoparticles have been characterized using UV-Vis spectrophotometer, TEM analysis. ...
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Green synthesis by plant extract has achieved a growth in interest. In the present study, zinc oxide nanoparticles were synthesized by olive extract.Morphological and structural properties of the synthesized zinc oxide nanoparticles have been characterized using UV-Vis spectrophotometer, TEM analysis. Then, the effect of zinc oxide nanoparticles at concentrations of 0, 200 and 400 ppm with pretreatment coronatine (0 and 50 nM) on the parameters of hydrogen peroxide and malondialdehyde and catalase (CAT) enzymes, ascorbate peroxidase (ASP), superoxide dismutase (SOD), phenylalanine ammonia lyase (PAL) was investigated. Synthesized zinc oxide nanoparticles were confirmed by maximum absorption at wavelength of 360 nm. TEM image revealed that zinc oxide nanoparticles were spherical with average size 41 nm. Investigation of zinc oxide nanoparticles on soybean showed that at concentrations of 400 ppm zinc oxide nanoparticles, enzyme activity of ASP, PAL, SOD, hydrogen peroxide and malondialdehyde increased. The content of malondialdehyde at a concentration of 200 ppm of zinc oxide nanoparticles did not change significantly compared to the control, but the activity of the SOD enzyme increased significantly compared to the control. Pretreatment coronatine improved stress at 200 ppm zinc oxide nanoparticles and reduced the content of hydrogen peroxide and malondialdehyde, but coronatine increased this content at 400 ppm zin oxide nanoparticles.
shahla hashemi; Fereshteh Mohamadhasani
Abstract
In the current study, the effect of different concentrations of cadmium (0, 200, 400μM) on root and shoot growth, malondialdehyde (MDA), total phenol, hydrogen peroxide (H2O2), chlorophyll and anthocyanin contents, phenylalanine ammonia lyase (PAL) activity were investigated in ...
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In the current study, the effect of different concentrations of cadmium (0, 200, 400μM) on root and shoot growth, malondialdehyde (MDA), total phenol, hydrogen peroxide (H2O2), chlorophyll and anthocyanin contents, phenylalanine ammonia lyase (PAL) activity were investigated in three varieties of soybean plant (Telar, DPX, Caspian). The results showed that Cd treatment caused reduction of root and shoot biomass, chlorophyll a content in all three cultivars with the highest reduction in Telar. Significant increase in total phenolics and anthocyanin contents and PAL activity was observed in all the three soybean cultivars with the Cd treatment. The content of MDA in all three cultivars was significantly increased in 400µM concentration of Cd treatment as compared to control. The results of this study indicated that Telar cultivar was the most sensitive cultivare to cadmium stress compared with Caspian and DPX cultivars.
shahla hashemi; Fereshteh Mohamadhasani
Abstract
Cadmium is a heavy metal and its high concentration has a high toxicity to plants and animals. Identification of Cd tolerant plants such as soybean, one of the most important oilseed crops, has a great importance. In this research, the effect of cadmium treatment (0, 200, 400μM) on growth of root, ...
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Cadmium is a heavy metal and its high concentration has a high toxicity to plants and animals. Identification of Cd tolerant plants such as soybean, one of the most important oilseed crops, has a great importance. In this research, the effect of cadmium treatment (0, 200, 400μM) on growth of root, shoot, malondialdehyde (MDA), total phenol, hydrogen peroxide (H2O2), chlorophyll a and anthocyanin contents, phenylalanine ammonia lyase activity were reported in three varieties of soybean plant (Telar, DPX, Caspian). The results showed that Cd reduced root and shoot growth, chlorophyll a content in Telar, DPX, Caspian that this reduction was more significant in Telar. Significant increase in total phenolics, anthocyanin contents and PAL activity of three varieties of soybean were also detected during Cd treatment.The highest content of phenol was observed at 400 μM cadmium concentration in Caspian and DPX varieties, but the highest content of anthocyanin was observed at 400 μm cadmium concentration in Telar, Caspian and DPX varieties. H2O2 content of Telar, DPX, Caspian varieties was increased by 16%, 7%, 9% for 400µM concentration of Cd compared to 200µM, respectively.The content of MDA in all three varieties was significantly increased in 400µM concentration of Cd treatment compared to control. The highest content of MDA was observed at 400 μm concentration in Telar variety. The results of this study indicated that Telar variety was more sensitive to cadmium stress in comparison with Caspian and DPX varieties. In other words, Caspian and DPX were the tolerant varieties against cadmium stress.
