zahra hajihassan; Navid Nazari
Abstract
Nowadays recombinant activin A has been produced in different expression hosts because of its vast clinical applications. As obtaining the highest cell density is one way to increase the production, optimization of cell culture medium is necessary. So in this study, glycerol and yeast extract concentration ...
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Nowadays recombinant activin A has been produced in different expression hosts because of its vast clinical applications. As obtaining the highest cell density is one way to increase the production, optimization of cell culture medium is necessary. So in this study, glycerol and yeast extract concentration as carbon and nitrogen sources of medium was optimized by using RSM (response surface methodology) in order to achieve the highest cell density of E. coli DE3) 21BL) strain transformed with pET21a:activin A vector. Furthermore, the effect of MgCl2 on the growth of mentioned bacterium was studied. The results showed that the highest cell density (growth) was achieved in 42.5 g/L of yeast extract, 22.5 g/L of glycerol and 30 mM of MgCl2. Also, dot blot results and data analysis with Image J in this study showed that the expression level of recombinant activin A in the optimized medium has been increased in comparison to standard cell culture medium.
