Mehrdad Zarafshar; Moslem Akbar nia; Sayed Mohsen Hosaini; Ali Sattarian; Maryam Niyakan
Abstract
Using of Nano-materials is one of the suitable ways for increasing of abiotic resistance in plants. In the current research, wild pear seedlings were irrigated with different concentrations of TiO2 and SiO2 NPs such as 0, 10, 100, 500 and 100 mgL-1. After this pretreatments, irrigation was stopped for ...
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Using of Nano-materials is one of the suitable ways for increasing of abiotic resistance in plants. In the current research, wild pear seedlings were irrigated with different concentrations of TiO2 and SiO2 NPs such as 0, 10, 100, 500 and 100 mgL-1. After this pretreatments, irrigation was stopped for 14 days to induce drought stress on the seedlings. At the end of the experiment, relative water content, xylem water potential, root biomass, electrolyte leakage rate, proline content and catalase enzyme activity were measured. The results showed that although both types of nanoparticles were differentially absorbed by roots of wild pear seedlings, the amelioration of water deficit in terms of all studied variables were observed for both of NPs with a slight differences between them. SiO2 NPs was more successful in improving root biomass and relative water content. On the other hand, TiO2 NPs was more successful in improving xylem water potential and catalase activity. Finally, amelioration effects of both NPs were clearly proved by the current research but further experiments are advised to find out involved mechanisms.
Mehrdad Zarafshar; Moslem Akbarinia; Hosain Asgari; Saeed mohsen Hosaini; mehdi rahaee
Abstract
One of the best solutions to overcome drought stress is the use of wild germplasm. We aim to understand how wild pear (Pyrus boisseriana) conserves water and recovers water potential after drought. After 18 days without water, which coincides with leaf rolling, we surveyed physiological and biochemical ...
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One of the best solutions to overcome drought stress is the use of wild germplasm. We aim to understand how wild pear (Pyrus boisseriana) conserves water and recovers water potential after drought. After 18 days without water, which coincides with leaf rolling, we surveyed physiological and biochemical parameters of pear seedlings and were re-surveyed them again after a week of re-watering. After signs of leaf rolling, the relative water content decreased to a critica level (35%).In contrast to the control, RWC declined by 58% and recovered acceptably. By the end of the experiment, xylem water potential declined -2.22 Mpa, but increased watering. For osmosis adjustment in the plant cell, free proline and glucose content was increased about 5-6%. The finding showed that increasing carotenoid content is one of the strategies in response to oxidative stress, because active oxygen species could not decrease chlorophyll content. On the other hand, MDA and H2O2 content as lipid peroxidation and oxidative stress indicator respectively narrowly increased. Finally it can be concluded that increasing the carotenoid and proline content are the important mechanism for water limiting stress. It seems that this wild pear germplasm can tolerate lack of water for about 18 days. Moreover, leaf rolling sign is a good indicator of drought resistance potential.
