Herbal
Seyedeh Zeynab Sharifsadat; Mahnaz Aghdasi; Faezeh Ghanati; Mohammad Hossein Arzanesh
Abstract
Introduction: Azospirillum is the nitrogen fixing microorganisms which improves the growth and development of cereals and other gramineae plants in association with their roots. The goal of present study was to investigate the effect of Azospirillum irakense inoculation in combinations with or without ...
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Introduction: Azospirillum is the nitrogen fixing microorganisms which improves the growth and development of cereals and other gramineae plants in association with their roots. The goal of present study was to investigate the effect of Azospirillum irakense inoculation in combinations with or without auxin (IAA) and/or gibberellin (GA3) treatment on the growth and biochemical parameters of rice (Oryza sative cv. hashemi) seedlings, as well as observing the bacterial colonization process in the root cells. Methods: The roots of 21-days-old rice seedlings were treated with different concentrations (0, 100, 200 ppm) of IAA and/or GA3 for 4 weeks after inoculation with Azosprillum. Result & discussion: The process of bacterial colonization involves the formation of a biofilm, disintegration of the wall, entry of bacteria into root parenchymal cells and development of isolated and scattered colonies. Dry and fresh weight of roots and aerial parts, total dry and fresh weight, and relative growth rate was significantly increased after inoculation with bacteria. The highest level of these parameters was obtained in the seedlings which inoculated with bacteria in combinations with IAA plus GA3 treatment. Meanwhile, the amount of hydrogen peroxide in the roots, soluble protein, soluble sugar and phosphorus was significantly increased in both roots and aerial parts of seedlings after inoculation with bacteria in combinations with IAA plus GA3 treatment, compared to the control. Conclusion: According to the current results, the application of Azospirillum irakense in combinations with IAA and GA3 treatment is a powerful strategy to improve rice seedlings growth
Herbal
morteza Mofid Bojnoordi; Mahnaz Aghdasi; Mohammad Fatemi
Abstract
Lactuca undulata Ledeb. is belongs to the family of Asteraceae. One of the most important compounds of this plant is chicoric acid. The present study aimed to investigate the impact of planting bed and conditions culture on cichoric acid production in different populations. For this purpose, collected ...
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Lactuca undulata Ledeb. is belongs to the family of Asteraceae. One of the most important compounds of this plant is chicoric acid. The present study aimed to investigate the impact of planting bed and conditions culture on cichoric acid production in different populations. For this purpose, collected seeds from regions (Cheshmeh Ali Damghan, Qom, Mirzabailo, Biarjamand and Firoozkooh) were planted in pots with two different kinds of soil (field soil and soil collected from natural habitat) and kept in either outdoor or growth chamber. The other group of seeds was planted in 1×2 m plots in the field. This experiment was conducted using completely randomized design. The current data revealed that the Mirzabailo population that was grown in the growth chamber had the highest growth rate compared to the other populations. Also, Cheshmeh Ali, Mirzabayloo and Firoozkooh entered the reproductive phase with a delay of one month compared to Qom and Biarjamand populations. Among the investigated populations, the amount of cichoric acid in plants grown in pots and outdoors was higher than the samples grown in the field or growth chamber. The highest amount of chicoric acid (1.24 mg/kg DW) was observed in Firoozkooh population which was grown in pots containing soil of the region. The highest amount of chlorogenic acid (0. 98 mg/g DW) and caffeic acid (0.50 mg/g DW) were also observed in Cheshmeh Ali population which grown in pots containing natural habit soil. The present results showed that there is high phytochemical diversity among the studied populations.
