Mohsen Mansouri
Abstract
Sesame (Sesamum indicum L.) belongs to the family (Pedaliaceae), and the crop is valuable. Sesame is an oilseed plant, and the study of its storage conditions is essential. Cryoprotectants are usually lower the freezing point of the solution and is blocked the creation of ice crystals in inside and outside ...
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Sesame (Sesamum indicum L.) belongs to the family (Pedaliaceae), and the crop is valuable. Sesame is an oilseed plant, and the study of its storage conditions is essential. Cryoprotectants are usually lower the freezing point of the solution and is blocked the creation of ice crystals in inside and outside solution of the cell. In this research was performed, cryopreservation to method vitrification with two types of solution protecting PVS2 and PVS3 in 7 times (20, 40, 60, 80, 100, 120 and 140 minutes). This experiment factorial was studied in the form of with completely randomized design (CRD) with two factors protective solution (two level) and time levels (seven level) and three replications. The results of analysis of variance for treatment time and soluble type in three traits, percentage germination, root and shoot length was significant at the level of 1% (P
Herbal
Mehdi Kakaei; Sahar Bakhtyiari; hojatolah mazaher laghab
Abstract
Background and purpose: cultivated alfalfa, which is known as green gold, is one of the best and most suitable fodder plants known in the world. Leaf weevil pest of alfalfa is considered as one of the limiting stresses in alfalfa production in the conditions of Iran. Material and Methods: In order to ...
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Background and purpose: cultivated alfalfa, which is known as green gold, is one of the best and most suitable fodder plants known in the world. Leaf weevil pest of alfalfa is considered as one of the limiting stresses in alfalfa production in the conditions of Iran. Material and Methods: In order to group pest tolerance in cultivated alfalfa, the traits of 30 alfalfa genotypes in the germplasm were evaluated in the training farm of Bu-ali Sina University. Findings: The interaction between the varieties and the levels of pest attack showed significant differences in relation to weevil number, fresh forage yield, dry forage yield, plant height at the time of harvesting at1% and the amount of damage at 5% probability. The number of larva had significant positive correlation at a level of 5% with damage amounts. In stepwise regression, damage percent as dependent and other traits as independent variables were used. The results showed that larvae number, leaf chlorophyll extent and plant height with 30/01 cumulative coefficient explained the most variations of damage percent. Conclusion: Considering to the percent of damage and the average comparison for other traits, Yazdi35 and Maoopa102 with the least number of larvae and the least percent of damage respectively and the highest height in attack or damage time as the most tolerance and cultivars Tak Buteh92 and MahalieMiandoab with the most larval number, damage percent and the lowest plant height were recognized as the most susceptible cultivars towards alfalfa weevil attack at damage time too.
Herbal
mehdi Kakaei; fatemeh hajmoradi; Mohsen Mansouri; mohama ali ebrahimi
Abstract
Background and purpose: Basil is annual herb it belongs to the mint family and it has 30 species; therefore, in this research, optimization of tissue culture conditions of purple basil plant for callus production, identification of callus induction percentage, growth rate, growth speed and relative water ...
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Background and purpose: Basil is annual herb it belongs to the mint family and it has 30 species; therefore, in this research, optimization of tissue culture conditions of purple basil plant for callus production, identification of callus induction percentage, growth rate, growth speed and relative water content of callus was done through tissue culture. Materials and Methods: The callus induction optimization experiment was carried out as a factorial in the form of a completely randomized design with 4 factors, micro samples and three levels of BA, IAA and IBA hormones. Findings: The results showed that the highest percentage of callus induction (88%) was related to the culture medium containing 2 mg/L IBA and 2 mg/L BA. The effect of hormonal compounds on the four investigated characteristics of callus generation percentage, growth speed, relative water content and growth rate (dry weight) has had a significant effect, which can be used to select the best combination in callus formation and tissue culture optimization, and finally the future goals of the breed. Also, the interaction effect of two explant factors and hormonal compounds only had a very significant effect (P≤1%) on the growth rate index. Conclusion: The quality of the induced calluses indicates the correct selection of explants and suitable hormonal compounds. Therefore, there is a direct relationship between callus production and growth regulators, and also according to the type of plant studied and the amount of hormone present in it, the amount of growth regulators used in callus induction will be different.
Mehdi Kakaei; Tayebeh Basaki
Abstract
Preservation of plant genetic resources is of particular importance. Using ultra-cold seed storage techniques, seeds can be stored for a long time at a much lower cost. In the present study, the cryopreservation of millet seeds by a verification method was investigated. The experiment in factorial format ...
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Preservation of plant genetic resources is of particular importance. Using ultra-cold seed storage techniques, seeds can be stored for a long time at a much lower cost. In the present study, the cryopreservation of millet seeds by a verification method was investigated. The experiment in factorial format (with two factors, the first factor in two levels of dewatering solution (PVS2 and PVS3) and the second factor in dewatering times in five- time levels (20, 40, 60, 80, and 100 minutes) and with a completely randomized design and it was done with three repetitions. The results of the analysis of variance showed that all traits (root length, stem length, and germination percentage) were significant in terms of treatment time at the level of 1% probability. Also, the type of solution was significant for root length and shoot length and the interaction effect of treatment time in solution type was significant only for shoot length at the level of 1% probability. Treatment of millet seeds with protective solutions for 60 minutes showed the highest germination rate compared to other treatments. The results showed that seeds treated with PVS3 solution had shorter stem length but longer root length than PVS2 solution. In general, cryopreservation can be considered as a suitable method to protect the germplasm of millet. PVS3 solution had the highest average germination percentage at 60 minutes, which is the best treatment composition for long-term storage of millet.
Herbal
Mehdi Kakaei; Mohsen Mansouri
Abstract
In this research was performed, cryopreservation to method vitrification with two types of solution protecting PVS2 and PVS3 in 7 times (20, 40, 60, 80, 100, 120 and 140 minutes). This experiment factorial was studied in the form of with completely randomized design (CRD) with two factors protective ...
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In this research was performed, cryopreservation to method vitrification with two types of solution protecting PVS2 and PVS3 in 7 times (20, 40, 60, 80, 100, 120 and 140 minutes). This experiment factorial was studied in the form of with completely randomized design (CRD) with two factors protective solution (two level) and time levels (seven level) and three replications. The results of analysis of variance for treatment time and soluble type in three traits, percentage germination, root and shoot length was significant at the level of 1% (P<0.01). Interaction between the time treatment in solution type for the length of root and shoot length were significant at the level of 1% (P<0.01) for germination percentage was insignificant at the level of 5% (P<0.05). Overall, analysis of variance showed that is effective the protective solution type and levels of treatment time in the process of vitrification and the cryopreservation Sesame plant in assessment indicators.
Mehdi Kakayi
Abstract
Spotted alfalfa aphid (Therioaphis trifolii) is an important pest of alfalfa. For study of proteome response to that aphid feeds, leaf sampling was made of alfalfa (Hamadani genotype) under treatment and control conditions. Leaf tissue proteome analysis identified 208 protein spots detectable in staining ...
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Spotted alfalfa aphid (Therioaphis trifolii) is an important pest of alfalfa. For study of proteome response to that aphid feeds, leaf sampling was made of alfalfa (Hamadani genotype) under treatment and control conditions. Leaf tissue proteome analysis identified 208 protein spots detectable in staining by Coomassie blue. Of these, according to t- test, 16 spots were significantly different at 1% (P≤ 0.01). The protein spots with significant expression changes, were searched by isoelectric point and molecular weight in databases and identified responsive possible proteins to pest stress conditions. Classification of identified proteins based on functional groups, showed that the highest of proteins change have been expressed in relation to stress, including protein disulfide isomerase, aldehyde dehydrogenase, heat shock proteins, polyphenol oxidase and proxiredoxins. The results revealed the activity of alfalfa at the level of protein expression to develop resistance to pest aphid spotted.
Mehdi Kakaei
Abstract
Different genetic plant materials, are valuable as potential reserves and the basis for plant breeding of specialists. The study of seed storage proteins, due to they have been less affected by environment and provide reliable information from genome genetic diversity of beans. In the present study, ...
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Different genetic plant materials, are valuable as potential reserves and the basis for plant breeding of specialists. The study of seed storage proteins, due to they have been less affected by environment and provide reliable information from genome genetic diversity of beans. In the present study, was conducted seed storage protein profiles of 12 phaseolus vulgaris genotypes based on laemmeli method using polyacrylamide gel electrophoresis systems (SDS-PAGE) in a 12.5 percent. Investigation the bands by use software NTSYS showed considerable differences. Minimum and maximum number of bands, was belong to genotype 11 (Shokofa) and genotype 12 (DF1083), respectively. Cophenetic coefficient was calculated to test the goodness of fit for cluster analysis with qualitative data that 0.89 is the appropriate fit is cluster analysis with qualitative data. Chart analysis of the main axes of the seed storage protein beans, cluster analysis, and compliance was approved five cluster. Thus according to genetic distances between the genotypes, we can use cross between Ks21193 and Shokofa for obtain the highest amount of heterosis in future breeding program. The highest and lowest amount of total protein, was associated with genotypes Dorsa and Shokofa, respectively.
