Pershia Mohamadzadeh; Shahlah Gamili
Abstract
Lead (Pb) is one of the destructive and harmful pollutants and it could have undesirable impact on tissues of organisms. In this study, the effect of various concentrations of Lead on liver and gill tissues were examined. The fingerling Rutilus rutilus were prepared and transferred to laboratory. ...
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Lead (Pb) is one of the destructive and harmful pollutants and it could have undesirable impact on tissues of organisms. In this study, the effect of various concentrations of Lead on liver and gill tissues were examined. The fingerling Rutilus rutilus were prepared and transferred to laboratory. After keeping the samples in aquarium, they were exposed to concentrations of 0.1, 0.2 and 0.4 mg/l Lead for 48, 96, 144 and 168 hours. At mentioned times, samples were removed from control and treatment aquariums then liver and gill tissues were extracted for damage determination. After staining, prepared slides were studied with magnification of 10 x and 40 x by optical microscope. In damaged tissues, Sinusoidal dilation, Vacuolization, Congestion, Hemorrhage, Nuclear piknosis, Hepatocytes necrosis, Hemosiderin accumulation in melanomacrophage cells and cellular acidophilic changes, Lymphocytic infiltration and focal necrosis of liver and gill, Mucosal hyperplasia, Secondary lamella and filaments edema, Pillar cells necrosis, Chloride cells necrosis, Congestion, Hemorrhage, Inflammatory cells infiltration, Hyperplasia, Hypertrophy, Secondary lamella adhesion, Mucosa accumulation, telangiectasia, Epithelial cells dysplasia and metaplasia to mucosal cells and Distal clubbing in secondary lamella were demonstrated (were observed). The results showed that, when the concentration of Lead Nitrate was increased more severe damages occurred. At 0.1 mg/l Lead, minimum tissue damages were observed, but with increasing time in 0.2 and 0.4 mg/l Lead maximum tissue injuries were found.
