jamshid fooladi; nashmin fayazi hossini; khadijeh kiarostami
Abstract
Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytic acid The major storage form of phosphorous in plants. Monogastric animals, such as pig, poultry and fish are not able to metabolize phytic acid. Adding phytase to diet monogastric animals lead ...
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Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytic acid The major storage form of phosphorous in plants. Monogastric animals, such as pig, poultry and fish are not able to metabolize phytic acid. Adding phytase to diet monogastric animals lead to increased avalibility of phosphorus. The objective of the present study is to isolate and characterize phytase producing bacterial strains from rhizosphere of wheat. Phytase production was determined by production of clear zones around the colonies on the sodium phytate containing medium and the best phytase producing strain was identified using molecular analysis. Also, the isolate was tested for P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) containing tricalcium phosphate as the sole P source. Among the 11 bacterial isolates, forming clear peripheral zones on a turbid agar plate, Staphylococcus pasteuri isolated able high potential for phytase production.This study showed that , Staphylococcus pasteuri capable production phytase and phosphatase enzyme.
