Aida Hamidkhani; Parisa Mohammadi; Ezzat Asgarani; Maryam Yoseffi
Abstract
Candida species are the most common cause of fungal infections. Early identification of Candida species is necessary for effective antifungal therapy and can also facilitate control of hospital infections. Identification of Candida species by conventional methods is time-consuming with low sensitivity, ...
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Candida species are the most common cause of fungal infections. Early identification of Candida species is necessary for effective antifungal therapy and can also facilitate control of hospital infections. Identification of Candida species by conventional methods is time-consuming with low sensitivity, but molecular approaches have provided an alternative way for their early diagnosis. Considering different molecular approaches limitation for diagnosis of Candida species, in this study PCR-DGGE was evaluated for detection of five different pathogenic Candida species including C.glabrata, C.tropicalis, C.albicans, C.orthopsilosis and C.parapsilosis. ITS2 region was amplified using ITS3-GC and ITS4 primer set and the amplified fragments were used in DGGE. The results showed that DGGE is capable of detection of Candida species being analyzed.
