Adel Javadi; Ezatallah Esfandiari; Alireza Pourmohammad
Abstract
In order to evaluate the effect of ascorbic acid and folic acid on germination parametersunder drought and salinity stress, two separate experiments was carried out in completelyrandomized factorial design with Azar2 Wheat cultivar. In this experiment, wheat seedswere pretreated for 16 hours with concentration ...
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In order to evaluate the effect of ascorbic acid and folic acid on germination parametersunder drought and salinity stress, two separate experiments was carried out in completelyrandomized factorial design with Azar2 Wheat cultivar. In this experiment, wheat seedswere pretreated for 16 hours with concentration of zero (control), 0.5 and 1 mM of ascorbic acid and 25 and 50 μM folic acid. Seeds were then dried at room temperature until theinitial weight. To apply drought and salinity stress the solution of polyethylene glycol 6000at 0 (control), -6 and -12 bar and sodium chloride at zero, 75 and 150 mM concentrationwere used, , respectively. Subsequently, petri dishes containing wheat seeds were kept attemperature of 24±1°C, 70% relative humidity and darkness condition for 8 days. After thementioned time, germination percentage, germination rate, root and shoot length andseedling dry weight were measured. The results showed that seed treatment with vitamins, even in favorable conditions, with reduction of the loss of seed reserves, can helpmore allocation of seed reserves at formation of seedling structure, and cause to generateof stronger seedlings. Under drought and salinity stress conditions, seed treatment withascorbic and folic acid, by creating appropriate conditions, can prevent damages caused bygenerate reactive oxygen species or prevent from their production, which the result of its,is the absent expression of oxidative stress on in seed germination. These factors result inincreasing growth respiration rate and formation of stronger seedlings. Therefore, increasing the amount of mentioned vitamins in seeds can improve the metabolic function, especially in difficult environmental situations and contribute in better seedling establishment.
