shahla siamaki; Siavosh Salmanzadeh-Ahrabi; Trouska Soufizadeh; Tahereh Falsafi; Mahvash Saifali; Jamshid Fooladi
Abstract
In this study colorimetric medium, which has been developed at Alzahra University, was compared with Quicolor (Salubris Co, USA) and Muller Hinton agar media. Antibacterial susceptibility patterns of 100 isolates of Enterobacteriaceae against 6 different antibiotics were determined by using disk diffusion ...
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In this study colorimetric medium, which has been developed at Alzahra University, was compared with Quicolor (Salubris Co, USA) and Muller Hinton agar media. Antibacterial susceptibility patterns of 100 isolates of Enterobacteriaceae against 6 different antibiotics were determined by using disk diffusion method. The agreement of these methods was defined by kappa analysis. Coefficient of variation (CV) was calculated by repeating antibacterial susceptibility test for 10 times using 2 standard strains and 2 clinical isolates of Enterobacteriaceae on three medium noted above. Kappa value was higher than 0.5 for all cases indicating a good or an excellent agreement of 3 media. ( CV) values were less than 0.05 in majority of cases showing a good reproducibility of testing media. According to the results of this study colorimetric medium can be used for rapid antibacterial susceptibility testing of Enterobacteriaceae.In terms of ease of functional and performance of this medium is similsr to foriegn and standard media.
Siavash Salman zadeh; Ehya Abdi ali; Zahra Boland ghamat pour
Abstract
Considering the importance of rapid determination of antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial agents and side-effects, and ...
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Considering the importance of rapid determination of antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial agents and side-effects, and lowers the overall healthcare costs.We have designed and evaluated a rapid antibacterial susceptibility medium which is based on a rapid colorimetric medium that indicates growth by changing its color.
In this study, we have assessed antibacterial susceptibility patterns of non-fermentative bacteria and Enterobacteriaceae isolated from clinical samples by rapid colorimetric medium according to standard CLSI method.
Colorimetric medium proved to be a reliable rapid test for determining antibacterial susceptibility testing, between two methods total agreement was 92.93% for Entrobacteriacea and 90.46% for non-fermentative bacteria. Major discrepancy was 3.28 % for non-fermentative bacteria and 3.52% for Enterobacreiacea. Colorimetric medium makes results available between 68- hours. It can be considered as a novel and reliable quick method for the assessing of bacterial susceptibility to antibiotics.
Zahra Bolandghamatpour
Abstract
Considering the importance of rapid determination of
antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate
therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial
agents and side-effects, ...
Read More
Considering the importance of rapid determination of
antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate
therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial
agents and side-effects, and lowers the overall healthcare costs.We have designed and evaluated a rapid
antibacterial susceptibility medium which is based on a rapid colorimetric medium that
indicates growth by changing its color. In this study, we have
assessed antibacterial susceptibility patterns of non-fermentative bacteria and
Enterobacteriaceae isolated from clinical samples by rapid colorimetric medium according to standard CLSI method. Colorimetric medium proved to be
a reliable rapid test for determining antibacterial susceptibility testing,
between two methods total agreement was 92.93% for Entrobacteriacea and 90.46%
for non-fermentative bacteria. Major discrepancy was 3.28 % for
non-fermentative bacteria and 3.52% for Enterobacreiacea. Colorimetric medium
makes results available between 6-8 hours. It can be considered as a novel and
reliable quick method for the assessing of bacterial susceptibility to
antibiotics.
