Siavash Salman zadeh; Ehya Abdi ali; Zahra Boland ghamat pour
Abstract
Considering the importance of rapid determination of antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial agents and side-effects, and ...
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Considering the importance of rapid determination of antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial agents and side-effects, and lowers the overall healthcare costs.We have designed and evaluated a rapid antibacterial susceptibility medium which is based on a rapid colorimetric medium that indicates growth by changing its color.
In this study, we have assessed antibacterial susceptibility patterns of non-fermentative bacteria and Enterobacteriaceae isolated from clinical samples by rapid colorimetric medium according to standard CLSI method.
Colorimetric medium proved to be a reliable rapid test for determining antibacterial susceptibility testing, between two methods total agreement was 92.93% for Entrobacteriacea and 90.46% for non-fermentative bacteria. Major discrepancy was 3.28 % for non-fermentative bacteria and 3.52% for Enterobacreiacea. Colorimetric medium makes results available between 68- hours. It can be considered as a novel and reliable quick method for the assessing of bacterial susceptibility to antibiotics.
Zahra Bolandghamatpour
Abstract
Considering the importance of rapid determination of
antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate
therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial
agents and side-effects, ...
Read More
Considering the importance of rapid determination of
antibacterial susceptibility of bacteria, isolated from infectious diseases for the selection of appropriate
therapy as soon as possible and increasing the success of therapy, decreasing unnecessary use of antibacterial
agents and side-effects, and lowers the overall healthcare costs.We have designed and evaluated a rapid
antibacterial susceptibility medium which is based on a rapid colorimetric medium that
indicates growth by changing its color. In this study, we have
assessed antibacterial susceptibility patterns of non-fermentative bacteria and
Enterobacteriaceae isolated from clinical samples by rapid colorimetric medium according to standard CLSI method. Colorimetric medium proved to be
a reliable rapid test for determining antibacterial susceptibility testing,
between two methods total agreement was 92.93% for Entrobacteriacea and 90.46%
for non-fermentative bacteria. Major discrepancy was 3.28 % for
non-fermentative bacteria and 3.52% for Enterobacreiacea. Colorimetric medium
makes results available between 6-8 hours. It can be considered as a novel and
reliable quick method for the assessing of bacterial susceptibility to
antibiotics.
Somaye Aazami; Ezzat Asgarani; Ahiya Abdi aali
Abstract
DNA extraction is the first stage of gene structure and function study, and finding an effective protocol for isolation DNA in genetic engineering and microbiology studies is essential. There are many different methods for DNA extraction which are interchangeable depending on the wall and membrane structure ...
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DNA extraction is the first stage of gene structure and function study, and finding an effective protocol for isolation DNA in genetic engineering and microbiology studies is essential. There are many different methods for DNA extraction which are interchangeable depending on the wall and membrane structure in the bacterium. Objective: The aim of this study was the assessment of different methods for DNA extraction from gram-negative bacterium (Pseudomonas aeruginosa) and the selection of effective protocol.Materials and methods: In this study three different methods of DNA extraction from gram-negative bacterium (Pseudomonas aeruginosa) is compared to determine the quantity and the quality of the DNA. These methods include lysis buffer containing proteinase K, DNGTM-plus kit and boiling. In each method, ten samples were selected in a completely random plan and the concentration of DNA was measured using spectrophotometer, Nanodrop and agarose gel electrophoresis. Result: Variance analysis illustrated a meaning difference between three methods. Also average comparison using Duncan test illustrated meaning difference. Finally, boiling method was considered as the most suitable method with the highest extraction efficiency.
