Naser Jafari; Mahdieh Faghani Panbezari; Abasalt Hosseinzadeh Colagar; Mohammadali Ebrahimzadeh
Abstract
Plantago major L. is one of the most important medicinal plants used in traditional medicine worldwide. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Plantago major was collected from different ...
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Plantago major L. is one of the most important medicinal plants used in traditional medicine worldwide. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Plantago major was collected from different regions of Mazandaran province, namely Shorab, Pain Kola, and Panbeh Zar Koti. Phenol and flavonoid levels from acidic methanol extract of plant samples were calculated using standard curve of Gallic acid and Quercetin, respectively. Antioxidant activity of the samples were investigated by three methods of DPPH, Nitric oxide and Reducing power. Our results showed that all of the Plantago major not only have a total phenol, flavonoid content, and antioxidant activity but also they have a biological activity of the extract depends on its phenol and flavonoid content. On the other hand, the antioxidant function of Plantago major species is affected by weather conditions as well as habitat characteristics and edaphic factors. On the basis of these findings, it is suggested that the identification of the potential of the Plantago major extract and selection of optimal phenological habitat and phases is essential and enables the extraction of effective ingredients in the applied of the products and the ability to enhance the medicinal yield of the plants.
Naser Jafari; Sayed Mobarakeh Hossaini; Mohamad Ali Ebrahimzadeh
Abstract
Euphorbia helioscopia L. is one of the important medicinal plants used in traditional medicine all over the world. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Euphorbia plant was collected ...
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Euphorbia helioscopia L. is one of the important medicinal plants used in traditional medicine all over the world. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Euphorbia plant was collected from 3 different altitudes (1861, 949 and 236 m) with 3 replications. The extract was then prepared using acidic methanol and its phenol content was calculated according to the standard curve of gallic acid and flavonoid based on the quercetin curve. Its antioxidant activity was also investigated in 3 methods of DPPH, nitric oxide and reducing power. This study has clearly shown that the biological activity of the extract depends on the amount of phenol and its flavonoids. The results of this study show that Euphorbia plant in Challufe region has the best antioxidant activity in the nitric oxide test and at the highest concentration (800 μg/ml) compared to the other two regions. Our results also showed that the ability of the extracts of Euphorbia in the three studied areas to inhibit nitric oxide free radicals was concentration dependent and increased with increasing concentration of their anti-radical activity. The results of this study showed that the species of Euphorbia had a high total phenol and flavonoid content and antioxidant activity. Which can be used as a medicinal plant species.
Naser Jafari; seyedeh mobarake hosseini kolaie; Mohammadali Ebrahimzadeh
Abstract
Euphorbia helioscopia L. is one of the important medicinal plants used in traditional medicine all over the world. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Euphorbia plant was collected from 3 different ...
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Euphorbia helioscopia L. is one of the important medicinal plants used in traditional medicine all over the world. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Euphorbia plant was collected from 3 different altitudes (1861, 949 and 236 m) with 3 replications. The extract was then prepared using acidic methanol and its phenol content was calculated according to the standard curve of gallic acid and flavonoid based on the quercetin curve. Its antioxidant activity was also investigated in 3 methods of DPPH, nitric oxide and reducing power. This study has clearly shown that the biological activity of the extract depends on the amount of phenol and its flavonoids. The results of this study show that Euphorbia plant in Challufe region has the best antioxidant activity in the nitric oxide test and at the highest concentration (800 μg/ml) compared to the other two regions. Our results also showed that the ability of the extracts of Euphorbia in the three studied areas to inhibit nitric oxide free radicals was concentration dependent and increased with increasing concentration of their anti-radical activity. The results of this study showed that the species of Euphorbia had a high total phenol and flavonoid content and antioxidant activity. Which can be used as a medicinal plant species.
Naser Jafari; Zahra Alavi; Mohammadali Ebrahimzadeh
Abstract
Algae are important aspects that can be extremely numerous applications in pharmaceutical, food, agricultural, medical uses and also used in wastewater treatment. In this study total phenolic and flavonoid contents of two types of green algae was performed by spectrophotometic and high performance liquid ...
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Algae are important aspects that can be extremely numerous applications in pharmaceutical, food, agricultural, medical uses and also used in wastewater treatment. In this study total phenolic and flavonoid contents of two types of green algae was performed by spectrophotometic and high performance liquid chromatography methods. In this study, Cladophora glomerata was collected from Caspian coast and estuaries of the Babolrood river and Spirogyra rivularis were collected from a pond adjacent rice paddies and livestock. Then extracted with acidified methanol produced a total phenolic compounds spectrophotometric method and HPLC method was used for the analysis of flavonoids in plants. Study of methanol extracts of four different regions of Cladophora and Spirogyra showed the greatest amount of phenolic compounds gallic acid according to Spirogyra algae in ponds and rice fields most of the flavonoid quercetin in terms of Cladophora algae in the estuary. According to the standard chromatogram retention time gallic acid (2.383), coumaric acid (3.817) and quercetin (7.217) was observed. So most of the time to prevent the inhibition of quercetin and gallic acid is less time. The two types of green algae rich in phenolic compounds and also have high antioxidant property so it can be used as a plant source of antioxidant compounds that can be used in food and pharmaceutical industries.
