Mohammad Yaghoobi Avini; Gholamhossein Ebrahimipour; Alireza Ghassempour
Abstract
The emergence of drug resistant pathogens indicatesthe need to discovery of new antibiotics. In the search for a new antibioticproducing microorganism, an Alcaligenes faecalis subsp. faecalis strain was isolatedfrom oilpolluted soils near Dezful. One factor at a time was used to find importantvariables ...
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The emergence of drug resistant pathogens indicatesthe need to discovery of new antibiotics. In the search for a new antibioticproducing microorganism, an Alcaligenes faecalis subsp. faecalis strain was isolatedfrom oilpolluted soils near Dezful. One factor at a time was used to find importantvariables in antimicrobial compound production and upon its result pH = 9,temperature 35 ˚C, NH4Cl 0.34%, sodium acetate 2% and K2HPO40.02% have greatest effect on production, respectively. Acetate and NH4Clconcentration, shaker revolution and fermentation time were selected foroptimization by response surface method with central composite design. The bestresult achieved by sodium acetate 1.88%, NH4Cl 0.29%, shaker 3 rpmand fermentation time 6.7 day which had 47% higher production than one factorat a time method. Results also indicated strong interaction between somevariables.
