Bahareh Zarei; Khadijeh Kiarostami; Monir Hosseinzade Namin; Mona Sorahinobar
Abstract
Silicon nanoparticles have distinctive physicochemical characteristics. They are able to enter into plants and impact the metabolisms of plants as well as improve plant growth and yield under unfavorable environmental conditions. This research was done in order to study the physiological effects of nanosilicon ...
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Silicon nanoparticles have distinctive physicochemical characteristics. They are able to enter into plants and impact the metabolisms of plants as well as improve plant growth and yield under unfavorable environmental conditions. This research was done in order to study the physiological effects of nanosilicon on Crocus sativus corm. The corms were treated with silicon nanoparticles in concentrations of 0, 9 and 18 mg L−1. The experiment was done as completely randomized design in three replicates. The results showed that nano-silicon treatment increased content of silicon, potassium, iron, zinc, magnesium and calcium contents in plants but did not affect the fresh and dry weight as well as protein content, significantly. Moreover, the number of daughter corms, malondialdehyde and proline content significantly increased in nano-silicon treated plants as compared to non-treated control. The maximum content of total phenolics and flavonoid content were observed in plants treated with 9 and 18 mgL-1 respectively. Based on the results, it can be concluded that the examined concentrations of silicon nanoparticles can increase saffron corm nutrient content, its capacity of antioxidant system by the production of secondary metabolites.
azadeh karimi.jafari; monir hosainzaadeh
Abstract
Saffron (Crocus sativus L.) is amember of Iridaceae family, that especially its stigma and corm are important in medicine and food. Today, silver nanoparticles with antimicrobial and fungal properties are used in many fields and also effects on ethylene receptors in plants. In Iran, due to increase saline ...
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Saffron (Crocus sativus L.) is amember of Iridaceae family, that especially its stigma and corm are important in medicine and food. Today, silver nanoparticles with antimicrobial and fungal properties are used in many fields and also effects on ethylene receptors in plants. In Iran, due to increase saline soils, study on reducing the effects of salinity stress by using nanosilver in plants can be a good perspective to control the effects of salinity stress on plants. For this purpose, an experiment was carried out using four treatments: nano silver (40 ppm), salinity (100 mM NaCl), nano silver (40 ppm) whit salinity (100 mM NaCl), control without any treatment and primary corm, with two irrigations and soaking methods were used. The amount of phosphorous, sodium and potassium ions of treated corms were investigated. Results showed that phosphorous ion in compared with control and primary corm increased in all stresses, which was significant at level (P≤0.05).The concentration of sodium in irrigations methods and potassium in soaking methods increased in salt stress (0.77 mg / gDW)(9.9 mg / gDW), but using salinity stress with nano silver (0.67 mg / gDW)(7.61 mg / gDW) showed a significant decrease in the levels of two mentioned ions (P≤0.05) compared with control(0.73 mg / gDW)(8.08 mg / gDW). In this study, the use of nanosilver particles with salt stress showed that the effects of salinity have been influenced and increased phosphorous ion and modified amount of sodium and potassium.
Arefeh Mohammadi Sanjani; Monir Hosseinzadeh; mona sorahi
Abstract
Silver nanoparticles are widely used in industry, medicine, biotechnology and agriculture. As a consequence, these nanoparticles are reaching the environment as waste products, which might have a negative impact on the environment, especially on plants. In this study, the effects of two concentrations ...
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Silver nanoparticles are widely used in industry, medicine, biotechnology and agriculture. As a consequence, these nanoparticles are reaching the environment as waste products, which might have a negative impact on the environment, especially on plants. In this study, the effects of two concentrations (5 and 7 ppm) of silver nanoparticles on safflower (Carthamus tinctorius) were investigated in vitro. Increased germination rate in from 70% (in non treated control) up to 90% (in 7 ppm nanosilver treated seeds) was bserved. 5% higher dry weight biomas in 5 ppm nanosilver treated plants and 12% lower biomas in 7% treated ones as compared to their non-treated controls confirmed that the toxicity threshold level is between these two concentrations. Increased thickness of epidermis layer and root hairs length as well as deletion of schloranchima cells in roots under silver nanoparticle was observed in anatomical study of safflower. The results also showed a remarkable decrease in and proline content in root while induction of MDA and proline content was observed in shoot under nanosilver treatment that can be an indicator for induction of oxidative stress in shoot by treatment. Moreover, nanosilver causes induction of chlrophyll a, total chlrophyll and carotenoid contenten in safflower.
Azadeh Karimi.jafari; Monir Hossein zadeh namil
Abstract
Saffron (Crocus sativus L.), a member of Iridaceae family, is valuable in pharmacies and food industries. Salinity is one of the growth limiting factors in saffron. Nano silver has many effects on plants ethylene receptors, chlorophylase activity and DNA, also reduces the stress effects in plants. The ...
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Saffron (Crocus sativus L.), a member of Iridaceae family, is valuable in pharmacies and food industries. Salinity is one of the growth limiting factors in saffron. Nano silver has many effects on plants ethylene receptors, chlorophylase activity and DNA, also reduces the stress effects in plants. The effects of nano silver (40 ppm) and salinity stress (100 mM NaCl) on physiological and biochemical properties of saffron corm with compare control (distilled water) were studied under greenhouse condition. The results showed that the number of fiber roots and Proline content in salinity stress plus nano silver and salinity without nano silver were increased but solute carbohydrate content was significantly decreased at level (P<0.05), compare to control. Length of root and carotenoids content in nano silver plus salinity treatment were decreased but dry weight of daughter corms were significantly increased compare with control at level (P<0.05). The results obtained from present study showed that nano silver increased plant defense against salinity stress.
Najme Sadat Azari; Monir Hosseinzadeh Namin; Mehrzad Roughani
Abstract
Saffron (Crocus sativus L.) is a member of Iridaceae family and beside its usefulness nutrition, it is considered to be a valuable plant in phytomedicine. This plant propagates bycorms. To evaluate the effect of corms aqueous extract on the human immune system, theaqueous extract was given to volunteer ...
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Saffron (Crocus sativus L.) is a member of Iridaceae family and beside its usefulness nutrition, it is considered to be a valuable plant in phytomedicine. This plant propagates bycorms. To evaluate the effect of corms aqueous extract on the human immune system, theaqueous extract was given to volunteer candidates as a soft drink. Blood samples were taken from candidates before and after administration. The candidates were divided into threegroups on the basis of drinks received between intervals of two blood samples e.g none(control), water alone and aqueous extract.. The analysis of IgG, IgM, C3 and C4 amounts inserum was performed by using appropriate SRID kit. The results showed that the amountsof C4, C3, IgG and IgM of control and water consumption groups slightly increased but thethird group who used corms aqueous extract showed that the concentration of C4, C3, IgGincreased while IgM decreased. This primary evaluation study revealed that the aqueousextract of corms stimulates human’s innate and humoral immune system.
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Abstract
Saffron (Crocus Sativus L.) is a member of Iridaceae family and one of the most valuable plants in medicine and nutrition point of view. This plant is propagated by corms. Corm is composed of many compounds including starch, proteins and components that have been involved in immune. To evaluate the effect ...
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Saffron (Crocus Sativus L.) is a member of Iridaceae family and one of the most valuable plants in medicine and nutrition point of view. This plant is propagated by corms. Corm is composed of many compounds including starch, proteins and components that have been involved in immune. To evaluate the effect of corms aqueous extract on the human immune system, the aqueous extract was used by candidates as soft drink. Blood samples were taken from candidates before and after treatments. The candidates were divided into three groups on the base of drink they got between intervals of two blood Sampling e.g. negative control( without any drinking), positive control (water drinking) and treatment (those who took aqueous extract of corm). The analysis of IgG, IgM, C3 and C4 were performed by using appropriate SRID kits. The results showed that the amount of C4, C3, IgG and IgM of control and water consumption groups were slightly increased but the third group who used aqueous extract showed increased concentrations of C4, C3, IgG and decreased IgM. The results suggested the aqueous extract of corms affected on human’s innate and humoral immune system.
Firuze Alirezayi; Khadijah Kiarostami; Monia Hussein Zadeh Namin
Abstract
Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants ...
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Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants secondry methabolites with high antioxidant properties ,silver nitrate as abiotic elicitor used in tissue culture of Lavandula angutifolia. Concentrations of 1, 2, 4 and 6 mg/l of silver nitrate as abiotic elicitor added to the one-month calli from leaf explant grown in MS medium with , Naphthaleneacetic acid(NAA)2mg/l, Benzyl amino purine (BAP) 4mg/l.After a month calli were harvested and the analyses were performed.The results showed that with increasing concentration of silver nitrate in the culture medium, growth of calli was increased and increasing the amount of silver nitrate concentration up to 4mg / l, increased Rosmarinic acid, flavonoids ,phenolic compounds and antioxidant activity .But in higher concentration of 4 mg / l the toxic effects of silver ions revealed and reduced the amount of Rosmarinic acid,phenolic compounds and antioxidant activity.
