Herbal
Erfaneh Shafiyon; Ali Akbar Ehsan Pour
Abstract
Tocopherols, identified as vitamin E, are amphipathic molecules known as antioxidant & able to remove ROS in lipophilic conditions. In this study, tobacco plants were cultured on MS medium containing concentrations of 0, 100, 200 mM NaCl supplemented with 0, 100, 200 & 400 mg.L ˉ1 α-tocopherol ...
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Tocopherols, identified as vitamin E, are amphipathic molecules known as antioxidant & able to remove ROS in lipophilic conditions. In this study, tobacco plants were cultured on MS medium containing concentrations of 0, 100, 200 mM NaCl supplemented with 0, 100, 200 & 400 mg.L ˉ1 α-tocopherol for 4 weeks. Then growth parameters including fresh & dry weight, photosynthetic pigments including chlorophyll a, b, total chlorophyll & carotenoids, sodium & potassium, proline, total phenol, soluble sugar, anthocyanins, & total protein were measured. The results showed that, increasing of α-tocopherolconcentrations increased fresh & dry weight of plants & the amount of photosynthetic pigments, proline, soluble sugar, total phenol, anthocyanins & potassium while, amount of sodium was decreased significantly.Protein content increased by the highest concentration of α-tocopherol. It seemed that, treatment with vitamin E by changing physiological parameters increased salt tolerance of tobacco plant.
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Abstract
salt stress has a an important role in deacreasing plant growth and development. Tocopherols, identified as vitamin E, are amphipathic molecules known as antioxidant and able to remove ROS in lipophilic conditions. In this study, tobacco plants were cultured on MS medium containig concentrations of 0, ...
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salt stress has a an important role in deacreasing plant growth and development. Tocopherols, identified as vitamin E, are amphipathic molecules known as antioxidant and able to remove ROS in lipophilic conditions. In this study, tobacco plants were cultured on MS medium containig concentrations of 0, 100, 200 mM NaCl supplemented with 0, 100, 200 and 400 mg.L ˉ1 of α-tocopherol for 4 weeks. Then growth parameters including fresh and dry weight, photosynthetic pigments including chlorophyll a, b, total chlorophyll and carotenoids, sodium and potassium, proline, total phenol, soluble sugar, anthocyanins, total protein were measured. The results showed that increasing of α-tocopherol concentrations increased fresh and dry weight of plants and the amount of photosynthetic pigments , proline, soluble sugar, total phenol, anthocyanins and potassium while, amount of sodium was decreased significantly. Protein content increased by the highest concentration of α-tocopherol. It seemed that treatment with vitamin E by changing physiological parameters resulted increasing of salinity tolerance of tobacco.
Aliakbar Ehsanpour; Mohamad Sadegh Maleki
Abstract
Salicylic acid is a plant hormone which accumulates ROS production like H2O2 as a signal molecule and in toxic concentration cause oxidative stress with hyper accumulation of ROS. Plants use enzymatic antioxidant like glutathione-ascorbate cycle enzymes and non-enzymatic antioxidant such as ASA and DHASA ...
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Salicylic acid is a plant hormone which accumulates ROS production like H2O2 as a signal molecule and in toxic concentration cause oxidative stress with hyper accumulation of ROS. Plants use enzymatic antioxidant like glutathione-ascorbate cycle enzymes and non-enzymatic antioxidant such as ASA and DHASA against oxidative stress. The effect of salicylic acid on ascorbate –glutathione cycle antioxidants in tomato plants as amodel plant is not clear Tomato (Lycopericon esculentum Mill) plants cultured in vitro for 4 weeks on MS medium containing salicylic acid (SA) treatment in concentrations of 0.0001, 0.001, 0.01, 0.1 and 0.5 mM SA.... In this study, the effect of salicylic acid investigated on growth parameters, H2O2 and MDA content and activity of SOD, CAT, APX, GR, DHAR enzymes and also ASA, DHASA, proline and total soluble sugar. The results showed that maximum amount of RWC, root and shoot length reached in 0.01 mM SA. H2O2 and MDA content increased under SA treatment. APX and CAT activity initially decrease in low concentrations of SA and then increased in toxic level (0.1 and 0.5 mM SA). Activity of GR, DHAR, SOD, ASA, DHASA, proline and total soluble sugar gradually increased compared with control plants. These results indicate that plants for defense against oxidative stress cause by ROS production change and induce proline, total soluble sugar and ascorbate-glutathione cycle enzymes and compounds. In this study, the concentration of 0.01 mM SA with the highest growth indices and the least amount of stress as the optimal concentration in the plant is introduced.
