Fariba Hasnvand; Masoud Haidarizadeh; Hamidreza Rezaee
Abstract
Dogs were domesticated from grey wolf about several thousand years ago with the beginning of agricultural activities . High genetic diversity and many varied breeds of domestic dogs are found in the world. Indigenous in Hamedan province try to cross bred between dogs and wolves in order to have ...
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Dogs were domesticated from grey wolf about several thousand years ago with the beginning of agricultural activities . High genetic diversity and many varied breeds of domestic dogs are found in the world. Indigenous in Hamedan province try to cross bred between dogs and wolves in order to have hybrid animals as guard dogs. The relationships between Hemedan`s dogs with the other Iranian ones have been investigated using mtDNA control-region sequence data from a subset of 55 individuals (29 dogs of Hamedan province and 26 individuals extracted from NCBI). Our results revealed an existence of high genetic diversity in Iranian dogs. Dogs in Hamedan Province have complex relationships and among them, observed several common haplotypes. The dogs in the province, have extensive communication with dogs in the other province, especially with northern area and western area. In fact, this communication complexity indicates behavior of chaos reproductive in the dog domestication.
Masuod Haidarizadeh; Fariba Hasanvand
Abstract
Cereal and legume kernels contain many α-amylase inhibitors which have the potential to inhibit the α-amylase activity from various sources. The subject of this study was the partial purification of α-amylase inhibitors of red bean seeds. To this end, extractions were carried out in ...
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Cereal and legume kernels contain many α-amylase inhibitors which have the potential to inhibit the α-amylase activity from various sources. The subject of this study was the partial purification of α-amylase inhibitors of red bean seeds. To this end, extractions were carried out in three parts with different saturation percentages of ammonium sulfate. Iin order to remove ammonium sulfate and monitor the inhibitory activity of the extracts on Aspergillus oryzae α-amylase, dialysis was carried out. By assaying the rate of inhibitory activity of each sample on the basis of their absorbance in 540 nm and comparison to absorbance of negative control, it was observed that only (30-60)% fraction of each extract had inhibitory activity on the α-amylase. Inhibitory effect of these fractions for red bean extract were calculated to be 89.32 %. For further characterization of these inhibitors, HPLC and SDS-PAGE analyses were carried out. By assaying the activity of these inhibitors on other α-amylases such as salivary and pancreatic α- amylases, it may be feasible to use these- amylase inhibitors similar to other inhibitors such as Acarbose and Miglitol, for the treatment of type II diabetes. The results may also be applicable in agriculture in the design of transgenic plants that express α-amylase inhibitors against α-amylases of pests.
Fariba Hasnvand; Masoud Haidarizadeh
Abstract
Cereal and legume kernels contain many α-amylase inhibitors which have potential to inhibit the α-amylase activity from variant sources. The subject of this study was partial purification of α-amylase inhibitors of red bean seeds. In this regard, we precipitated extractions as three ...
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Cereal and legume kernels contain many α-amylase inhibitors which have potential to inhibit the α-amylase activity from variant sources. The subject of this study was partial purification of α-amylase inhibitors of red bean seeds. In this regard, we precipitated extractions as three parts with different saturation percentages of ammonium sulfate. Then, we dialyzed, in order to remove ammonium sulfate and better checking of their inhibitory activity upon on Aspergillus oryzae α-amylase. By assaying rate of inhibitory activity of each sample on the basis of their absorbance in 540 nm and comparison to absorbance of negative control, we saw that only (30-60)% fraction of each extract had inhibitory activity upon on defined α-amylase. Inhibitory of these fractions for red bean extract were 89.32 percentage. For more characterization of these inhibitors, we conducted HPLC and SDS-PAGE analyses. By assaying the activity of these inhibitors upon on other α-amylase such as salivary and pancreatic α-amylases, may be it is possible to use these α- amylase inhibitors such as Acarbose and Miglitol for treatment of type II diabetes. Also the results might be applied in agriculture to produce transgenic plants that express α-amylase inhibitors against α-amylase of pests.
