بررسی شاخص‌های فیزیولوژیکی و بیوشیمیایی برخی رقم‌های سویا تحت تنش کادمیوم

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان، کرمان، ایران

2 مربی، گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران

10.22051/jab.2020.4421

چکیده

کادمیوم در زمره فلزات سنگین می‌باشد و غلظت بالای آن سمیت زیادی برای گیاهان و حیوانات دارد. شناسایی گیاهان مقاوم به کادمیوم از جمله سویا، که یکی از گیاهان مهم روغنی محسوب می‌شود از اهمیت بالایی برخوردار است. در این پژوهش، اثرات تیمار کادمیوم (0، 200 و 400 میکرومولار) بر رشد ریشه و ساقه و محتوای مالون‌دی-آلدئید، فنل‌کل، پراکسید‌هیدروژن، کلروفیل، آنتوسیانین و فعالیت آنزیم فنیل آلانین آمونیالیاز (PAL) در سه رقم تلار، کاسپین، DPX گیاه سویا گزارش شد. نتایج نشان داد که تیمار کادمیوم باعث کاهش پارامترهای رشد ریشه و ساقه، کلروفیل در تلار، کاسپین، DPX شد که این کاهش در تلار چشمگیرتر بود. میزان آنتوسیانین، فنل و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز در تیمار گیاهان با کادمیوم در سه رقم سویا افزایش یافت. بیشترین محتوای فنل در تیمار 400 میکرومولار کاسپین و DPX بود اما بیشترین محتوای آنتوسیانین در تیمار 400 میکرومولار تلار، کاسپین و DPX مشاهده شد. محتوای پراکسید‌هیدروژن در تیمار کادمیوم 400 میکرومولار در ارقام تلار، DPX و کاسپین نسبت به تیمار200 میکرومولار به‌ترتیب 16، 7، 9 درصد افزایش یافت. محتوای مالون‌دی‌آلدئید در هر سه رقم در تیمار 400 میکرومولار کادمیوم نسبت به شاهد، افزایش معنی‌داری دیده شد. بالاترین محتوای مالون‌دی‌آلدئید نیز در تیمار 400 میکرومولار کادمیوم در رقم تلار مشاهده گردید. نتایج این تحقیق نشان داد که رقم تلار تحت تنش کادمیوم حساسیت بیشتری نسبت به رقم‌های کاسپین، DPX دارد. به‌عبارت دیگر رقم‌های کاسپین و DPX مقاومت بالاتری در مقابل سمیت کادمیوم دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Study of physiological and biochemical parameters of some soybean (Glycine max) varieties (Telar, DPX, Caspian) under cadmium stress

نویسندگان [English]

  • shahla hashemi 1
  • Fereshteh Mohamadhasani 2

1 Biology Department, Faculty of Science, Shahid Bahonar University of Kerman, Iran

2 Instructor, Department of Biology, Payame Noor University, Tehran, Iran

چکیده [English]

 
In the current study, the effect of different concentrations of cadmium (0, 200, 400μM) on  root and shoot growth, malondialdehyde (MDA), total phenol, hydrogen peroxide (H2O2), chlorophyll  and anthocyanin contents, phenylalanine ammonia lyase (PAL) activity were investigated in three varieties of soybean plant (Telar, DPX, Caspian). The results showed that Cd treatment caused reduction of root and shoot biomass, chlorophyll a content in all three cultivars with the highest reduction in Telar. Significant increase in total phenolics and anthocyanin contents and PAL activity was observed in all the three soybean cultivars with the Cd treatment. The content of MDA in all three cultivars was significantly increased in 400µM concentration of Cd treatment as compared to control. The results of this study indicated that Telar cultivar was the most sensitive cultivare to cadmium stress compared with Caspian and DPX cultivars.

کلیدواژه‌ها [English]

  • cadmium
  • soybean
  • H2O2
  • anthocyanin
  • Phenol

 

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