اثر تیمار پاکلوبوترازول بر برخی شاخص‌های فیزیولوژیکی موثر در تحمل تنش سرما در جوانه انگور رقم بیدانه سفید

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی دانشکده علوم دانشگاه ملی ملایر

2 دانشیار گروه مهندسی علوم باغبانی و فضای سبز دانشکده کشاورزی دانشگاه ملایر

3 استادیار گروه زیست شناسی دانشکده علوم دانشگاه ملایر.

چکیده

 



هدف این پژوهش بررسی اثر پاکلوبوترازول بر شاخص‌های فیزیولوژکی جوانههای بهاری انگور بود. محلول پاشی پاکلوبوترازول روی رقم بیدانه سفید با غلظت های0، 125، 250، 500 و 1000 میلی گرم درلیتر در سه تکرار (بلوک) در قالب طرح بلوک کامل تصادفی در اواخر دوره رکود جوانه ها در ملایر انجام شد. تغییرات شاخص های محتوای پرولین، مالون دی آلدهید، قندمحلول، پروتئین کل، رنگیزه های فتوسنتزی و فعالیت آنزیم های پراکسیداز، پلی فنل-اکسیداز و آسکوربات پراکسیداز جوانه ها در بهار مورد بررسی قرار گرفت. نتایج اثر معنی دار و مطلوب پاکلوبوترازول را روی شاخص های پرولین، قندمحلول، پروتئین کل و آنزیم ها نشان داد. تیمار 500 میلی گرم درلیتر بیشترین اثر را در کاهش میزان مالون دی آلدهید جوانه های برگ (13 درصد کاهش نسبت به شاهد) و افزایش فعالیت آنزیم های پراکسیداز، پلی فنل اکسیداز و آسکوربات پراکسیداز (بترتیب با مقادیر 563، 478 و 407 درصد افزایش نسبت به شاهد)، نشان داد. با توجه به تاثیرهای شاخص های مزبور در تعیین میزان تحمل به تنش‌های محیطی، غلظت 500 میلی گرم درلیتر بعنوان مناسب ترین غلظت در ارتقا سازش پذیری جوانه ها برای تحمل سرمای دیررس بهاره مشخص گردید.

عنوان مقاله [English]

The treatment effect of Paclobutrazol on some effective physiological traits of cold stress tolerance in bud grape of cv. Bidane Sefide

نویسندگان [English]

  • parvaneh roostaei 1
  • mousa rasouli 2
  • Arash Babaei 3

1 Biology Departement, Faculty of Sciences, Malayer University, Malayer, Iran

2 Associate Professor of Horticultural Science and Land scape Engineering Departement, Faculty of Agriculture, Malayer University, Malayer, Iran

3 Assistant Professor of Biology Department, Faculty of Sciences, Malayer University, Malayer, Iran

چکیده [English]

Propose of this study was investigation on the effect of paclobutrazol on physiological parameters of spring buds of grapes. Spraying of paclobutrazol was carried out on cultivar of Bidane Sefide at concentrations of 0, 125, 250, 500 and 1000 mg/L with three replicate contest of the randomized complete block design (CRB) in late buds dormancy in Malayer. Chosen in of proline, malondialdehyde, soluble sugar, total protein, photosynthesis pigments and enzymes activity of peroxidase, polyphenol oxidase and ascorbate peroxidase of buds were measured in spring. Results were shown significant effect of paclobutrazol on parameters of proline, soluble sugar, total protein and mentioned enzymes. Treatment of 500 mg/L had the most effect on reduced of malondialdehyde levels (with 13% decrease compared to the control) and increased the activity of peroxidase, polyphenol oxidase and ascorbate peroxidase (with 563, 478, 407 decrease compared to the control, respectively). Due to the effects of these parameters on determining tolerance to environmental stresses, concentration of 500 mg/L was determined as best concentration to improve the adaptability of buds for late cold spring tolerance

کلیدواژه‌ها [English]

  • antioxidant enzymes
  • Malondialdehyde
  • Proline
  • soluble sugar
  • Total protein

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