اثر ویتامین E (آلفا توکوفرول) بر برخی از شاخص‌های فیزیولوژیکی و بیوشیمیایی گیاه تنباکوNicotiana rustica L.)) تحت تنش شوری در کشت در شیشه

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد، گروه زیست شناسی، دانشکده علوم، دانشگاه اصفهان، اصفهان

2 استاد، گروه زیست شناسی، دانشکده علوم، دانشگاه اصفهان، اصفهان

چکیده

توکوفرول‌ها که تحت عنوان ویتامین Eشناخته می‌شوند، مولکول‌های آمفی‌پاتیکی هستند که به عنوان آنتی‌اکسیدان قادر به حذف گونه‌های فعال اکسیژن می‌باشند. در این پژوهش، گیاه تنباکو به مدت چهار هفته در محیط MS حاوی غلظت‌های صفر، 100 و 200 میلی‌مولار NaCl به همراه غلظت‌های، 100، 200 و 400 میلی‌گرم بر لیتر ویتامین E رشد داده شد و سپس شاخص‌های رشد از جمله وزن تر و خشک, میزان رنگیزه‌های فتوسنتزی، شامل کلروفیل a، b، کلروفیل کل، کاروتنوئیدها، غلظت­های سدیم و پتاسیم، میزان پرولین، ترکیبات فنلی ، قند محلول، آنتوسیانین و پروتئین کل، مورد بررسی قرار گرفت. نتایج نشان داد که افزایش غلظت آلفاتوکوفرول سبب افزایش معنی‌دار وزن تر و خشک گیاهو افزایش میزان رنگیزه‌های فتوسنتزی و نیز پتاسیم و کاهش سدیم گردیده است. علاوه بر این، تیمار آلفاتوکوفرول سبب افزایش میزان پرولین، ترکیبات ترکیبات فنلی کل، قند محلول و آلفاتوکوفرول داخلی گیاه در تنش شوری شد. همچنین میزان پروتئین گیاه فقط توسط بالاترین غلظت آلفاتوکوفرل افزایش یافت. به نظر می‌رسد تیمار ویتامین E با تغییر برخی از شاخص‌های فیزیولوژیکی منجر به افزایش تحمل به شوری گیاه تنباکو گردیده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of vitamin E (alpha-tocopherol) on some physiological & biochemical parameters of tobacco plant (Nicotiana rustica L.) under in vitro salt stress

نویسندگان [English]

  • Erfaneh Shafiyon 1
  • Ali Akbar Ehsan Pour 2

1 M.Sc., Department of Biology, Faculty of Science, University of Isfahan, Isfahan  

2 Professor, Department of Biology, Faculty of Science, University of Isfahan, Isfahan

چکیده [English]

Tocopherols, identified as vitamin E, are amphipathic molecules known as antioxidant & able to remove ROS in lipophilic conditions. In this study, tobacco plants were cultured on MS medium containing concentrations of 0, 100, 200 mM NaCl supplemented with 0, 100, 200 & 400 mg.L ˉ1 α-tocopherol for 4 weeks. Then growth parameters including fresh & dry weight, photosynthetic pigments including chlorophyll a, b, total chlorophyll & carotenoids, sodium & potassium, proline, total phenol, soluble sugar, anthocyanins, & total protein were measured. The results showed that, increasing of α-tocopherolconcentrations increased fresh & dry weight of plants & the amount of photosynthetic pigments, proline, soluble sugar, total phenol, anthocyanins & potassium while, amount of sodium was decreased significantly.Protein content increased by the highest concentration of α-tocopherol. It seemed that, treatment with vitamin E by changing physiological parameters increased salt tolerance of tobacco plant.
 

کلیدواژه‌ها [English]

  • α-tocopherol
  • Growth parameters
  • Salt stress
  • Tobacco plant
 

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