اثر الیسیتورها و ترکیب فیتوهورمون‌ها بر رشد، محتویات رنگیزه‌های فتوسنتزی و پروتئین جلبک sorokiniana Chlorella

نوع مقاله: مقاله پژوهشی

نویسندگان

1 - مربی ، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور تهران، ایران

2 - دانشیار ، گروه کشاورزی، دانشکده کشاورزی، دانشگاه پیام نورتهران، ایران

3 دانشیار ، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران

4 استاد، گروه کشاورزی، دانشکده کشاورزی، دانشگاه پیام نورتهران، ایران

5 استادیار ، گروه شیمی، دانشکده علوم پایه، دانشگاه پیام نورتهران، ایران

چکیده

جلبک کلرلا کاربرد دارویی، صنعتی وغذایی دارد. به همین دلیل بالا بردن بیوماس ومتابولیت‌های آن ارزشمند است. هدف این پژوهش نیز به دست آوردن بهترین ماده یا ترکیب هورمونی برای افزایش بیوماس ومتابولیت‌هایارزشمند Chlorella sorokiniana بود. در این پژوهشجلبک C. sorokiniana در محیط کشت پایۀBold  ((BBM اصلاح‌شدهکشت شد. به محیط‌ها نسبت‌های مختلف هورمونی نفتالن استیک اسید ((NAA، ایندول بوتیریک اسید ( (IBAوکینتین Kin) )، نیترات و فسفات و تیامین پیروفسفات افزوده شد. در تیمار با NAA (10 میلی‌گرم در لیتر)  Kin+(یک میلی‌گرم در لیتر) وزن خشک، محتویات رنگیزه‌های فتوسنتزی و پروتئین نسبت به شاهد افزایش معنی‌داری یافت. این صفات در تیمار با IBA+Kin وتیامین پیروفسفات اختلاف معنی‌داری را با شاهد نشان نمی‌داد بالاترین افزایش معنی دار وزن خشک، محتویات رنگیزه‌های فتوسنتزی و پروتئین نسبت به شاهد در تیمار افزایش نیترات و فسفات مشاهده شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of elicitors and combined phytohormones on growth , photosynthetic pigments and protein contents of Chlorella sorokiniana

نویسندگان [English]

  • Amaneh Jamshidi 1
  • MohamadAli Ebrahimi 2
  • Tayebeh Rajabiyan 3
  • GhoulamReza Bakhshi Khaniki 4
  • Shahla Mouzafari 5

1 - Instructor, Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran

2 - Associate Professor, Department of Agriculture, Faculty of Agriculture, Payam Nourtahan University, Iran

3 Associate Professor, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

4 Professor, Department of Agriculture, Faculty of Agriculture, Payame Nourtahan University, Iran  

5 Assistant Professor, Department of Chemistry, Faculty of Basic Sciences, Payam Nourtahan University, Iran

چکیده [English]

Chlorella has applications in medicine, industry and food. For this reason, increase of biomass and its metabolites is valuable. Aim of this study was to obtain the best substance or phytohormone combinations to increase biomass and metabolites contents of Chlorella sorokiniana. In this study, Chlorella sorokiniana was cultured in modified Bold Basal Medium (BBM). Different proportions of plant hormones, naphthalene acetic acid (NAA), indolebutyric acid (IBA) and kinetin (Kin), nitrate and phosphate and thiamine pyrophosphate were added to medium. There was significant rise in dry weight, photosynthetic pigments and protein contents in treatment NAA (10mg.L-1) + Kin (1mg.L-1) compared with control. In treatment of IBA+ Kin, and thiamine pyrophosphate no significant difference was observed in these parameters. The highest significant enhancement of dry weight, metabolites and protein contents were observed in medium supplemented with higher amount of nitrate and phosphate.   
                        

کلیدواژه‌ها [English]

  • alga
  • biomass
  • nitrate and phosphate
  • plant hormone

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