ارتباط تولید هورمون IAA با تنش های خشکی و شوری و تغییرات غلظت تریپتوفان در باکتری های جداسازی شده از خاک های شور و شور-سدیمی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی ایلام، سازمان تحقیقات، آموزش و ترویج کشاورزی، ایلام، ایران

2 استاد شیمی خاک، دانشگاه تهران، ایران

3 استاد بیولوژی و بیوتکنولوژی خاک، دانشگاه تهران، ایران

4 استاد موسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

با توجه به اهمیت تولید IAA توسط باکتری های خاک، به ویژه نقش آن در القاء ریشه زایی در خاک های متأثر از خشکی و شوری این تحقیق انجام شد. در این تحقیق، ضمن جداسازی 400 باکتری از خاک های با درجات مختلف EC و SAR، تغییرات تولید IAA و اثر مقادیر مختلف تریپتوفان و تنش های خشکی و شوری بر تولید این هورمون تجزیه و تحلیل شد. نتایج نشان داد که اثر تنش های خشکی و شوری بر تغییرات تولید IAA از نظر آماری معنی دار بود. گونه باکتری siccitoleransArthrobacter که از خاک های شور-سدیمی جداسازی شد، مولد هورمون IAA بوده و نسبت به سایر جدایه های باکتری دارای بیشترین پایداری تولید در تنش های خشکی و شوری بود. همچنین این باکتری، متحمل به خشکی و شوری بوده و به تا EC برابر با 40 دسی زیمنس بر متر و پتانسیل اسمزی 25- بار همچنان به تولید IAA ادامه داد. این باکتری، کارایی بالایی در مصرف تریپتوفان در شرایط تنش های خشکی و شوری از خود نشان داد. به طوری که مقدار تولید هورمون IAA به ازاء مصرف هر واحد تریپتوفان، برابر با 1/0 بود  و به عنوان کاراترین باکتری، گزارش شد. این باکتری با توجه به آزمون های انجام شده و برتری نسبی آن، برای انجام آزمون های مزرعه ای در تنش های موجود در خاک های متأثر از خشکی، شوری و شور- سدیمی، پیشنهاد شد.

کلیدواژه‌ها

عنوان مقاله [English]

Relation of IAA production with drought and salinity tensions and tryptophan variability in isolated bacteria from saline and saline-sodic soils

نویسندگان [English]

  • Reza Soleimani 1
  • Hasan Towfighi 2
  • Hossein Ali Alikhani 3
  • kazem khavazi 4

1 Assistant Professor, Agricultural and Natural Sciences Research Center, Ilam, Research Organization, Education and Promotion of Agriculture, Ilam, Iran

2 Professor of Soil Chemistry, University of Tehran, Iran

3 Professor of Biology and Soil Biotechnology, University of Tehran, Iran

4 استاد موسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده [English]

This investigation was carried out because of the importance of IAA production by bacteria and its role in rooting induction in drought, saline and saline-sodic soils., especially.In this investigation, 400 bacteria were isolated from soils with different levels of electrical conductivity (EC) and sodium adsorption ratio (SAR) and analyzed the variability of their ability in IAA production and the effects of different amounts of tryptophan and drought-salinity tensions on IAA production.Results indicated that the effects of drought and salinity tensions on variability of IAA production were significant, statistically. One of bacterial species as Arthrobacter siccitolerans that was isolated from saline-sodic soils, was IAA producer and had the highest dtability in tensions. Also, this bacterium was osmotolerant and halotolerant and continued to IAA production up to EC of 40 dS.m-1 and OP (Osmosis pressure) of -20 bar. A. siccitolerans has a high efficiency in tryptophan consumption in tension conditions. So, IAA production per unit of consumed tryptophan was 0.1, and reported as the most efficient bacterium in drought-salinity conditions. So, According to the tests on isolates, A. siccitolerans was proposed for field experiments in drought, salinity and salinity-sodicity affected soils.

کلیدواژه‌ها [English]

  • Arthrobacter siccitolerans
  • IAA
  • Salinity
  • Salinity-sodicity
  • Tryptophan

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