شناسایی 30 جدایه قارچ Metarhizium با استفاده از توالی ITS1-5/8S-ITS2

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم و زیست فناوری گیاهی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، اوین،تهران

2 دانش آموخته کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشگاه صنعتی شاهرود

3 استادیار، گروه زراعت و اصلاح نباتات، دانشگاه صنعتی شاهرود، شاهرود

4 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شاهرود

5 گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه نورث وست چین

چکیده

Metarhizium spp قارچبیمارگریاستکهدرکنترلزیستیطیف وسیعیازآفتهانقشداردوبرپایهویژگیهایتبارشناختی وملکولیشناساییمیگردد. توالیهایITS و5/ S8 ریبوزومی، برایشناساییگونههاوسویههای 30 جدایهیجمعآوریشده ازخاکهای شاهرودوشمالایرانبههمراه 10 توالیازگونه هایشناختهشدهبرایرسمدرختتبارشناختیمتارایزیومبه کاربردهشدند. صفاتنرخرشد،زهرآگینیوتعدادکنیدیهای تولیدیجدایههارویمحیطکشتPDA حاویقارچکشهای انتخابیاندازهگیریشدند. سوسپانسیون 1 ×108 کنیدی/میلیلیتر اینجدایههاجهتتاییدکنترلزیستیآنها،رویحشرهیکامل سوسکقرمزآرد(Tribolium castaneum (Col, Tenebrionidaeتیمارشد. نتایجنشاندادکهبالاترینمیزاننرخرشدبهجدایههای،SHU.J.11،SHU.J.35،SHU.J.22 ،SHU.J.33،SHU.J.27،SHU.J.33 ،SHU.J.22،SHU.J.11،  SHU.J.02،SHU.J.29   SHU.J.24 تعلقداشت. 10 جدایهبر اساسطبقهبندیYip ،درسطحA باقدرتتهاجمیبالاعلیه فتمیزبان،قرارگرفتند کهدراینبینجدایهیSHU.J.01 با میانگینتولید 8/025 × 107 کنیدی،برطبقگروهبندیپتلامول پراسرتسان،بهعنوانمهاجمترینجدایهجهتکنترلزیستی آفتشناساییشد. ضریبهمبستگیمعنیدار(P<0.01)صفاتنیزنشاندادکهجدایههایداراینرخرشدسریعتر،باتولیدتعدادکنیدیبیشتر،ازقدرتزهرآگینیبیشتریدرآلودهسازیمیزبانبرخوردارندوازنظرکاربردیمیتواننددرکنترلزیستیآفات،بطورموفقمورداستفادهقرارگیرند.
 

کلیدواژه‌ها

عنوان مقاله [English]

Identification of 30 isolates of the Metarhizium spp. via ITS1-5 / 8S-ITS2 sequence

نویسندگان [English]

  • nasser farokhy 1
  • mahsa jokar 2
  • mahdiye Parsaeiyan 3
  • ali Derakhshan Shadmehri 4
  • abolfazl Masoudi 5

1 Assistant Professor, Department of Plant Sciences & Biotechnology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University G.C., Evin, Tehran

2 MSc. in Plant Agricultural Biotechnology, Department of Agronomy & Plant Breeding, Shahrood University of

3 Assistant Professor, Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Shahrood

4 Assistant Professor, Department of Horticulture & Plant Protection, Faculty of Agriculture, University of Shahrood

5 Ph.D student in Biotechnology, Northwest A & F University, China

چکیده [English]

Metarhizium spp., an enthemopathogenic fungus, are usually recognized on the basis of their morphological and molecular characteristics. ITS and S8/5 ribosomal sequences used to identify 30 isolates collected from soils of Shahrood and northern parts of Iran, along with 10 sequences from known species to establish a phylogenetic relationship. Growth rate and conidia production were measured on PDA containing selected fungicide. Suspensions of 108 conidia per ml of the isolates were used against red flour beetle (Tribolium castaneum (Col, Tenebrionidae)) adults to assess the biocontrol efficacies of each isolate. The highest growth rates belonged to SHU.J.11, SHU.J.35, SHU.J.22, SHU.J.33, SHU.J.27, SHU.J.24, SHU.J.02 and SHU .J.29. According to Petlamul classification, SHU.J.01 with an average production of 8.025 × 107 was the highest and according to Yip classification, 10 isolates were considered as A-level. Pearson correlation coefficient for the three parameters was statistically significant at 1%. Accordingly, the isolate with greater growth rate, produced more conidia and had greater efficacy in inoculating the host.

کلیدواژه‌ها [English]

  • Biocontrol
  • ITS1-5/8S-ITS2
  • Tribolium castaneum
  • Metarhizium

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