افزایش پراکسیدهیدروژن و فعالیت آنزیم‌های آنتی‌اکسیدانت در سلول‌های خشخاش ایرانی تیمار شده با متیل جاسمونات و سالیسیلیک اسید

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشگاه محقق اردبیلی، اردبیل

2 دانشیار،عضو هیات علمی گروه زراعت و اصلاح نباتات- دانشکده علوم کشاورزی- دانشگاه محقق اردبیلی

3 دانشیار،گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل

4 استادیار،گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل

چکیده

سالیسیلیک اسید و جاسمونات‌ها از تنظیم کننده های رشدی هستند که در مکانیسم‌های دفاعی گیاهان در برابر تنش‌های زیستی و غیرزیستی و تولید متابولیت‌های ثانویه نقش مهمی دارند. در این تحقیق تأثیر متیل جاسمونات و سالیسیلیک اسید بر میزان پراکسیدهیدروژن، پرولین و پروتئین کل و فعالیت آنزیم‌های آنتی اکسیدان مورد بررسی قرار گرفت. کشت سوسپانسیون سلولی خشخاش ایرانی در محیط MS مایع حاوی 2,4-D، کینتین و آسکوربیک اسید انجام گردید. سلول‌ها تحت تأثیر تیمار های متیل جاسمونات (100 میکرومولار) و سالیسیلیک اسید (75 میلی گرم بر لیتر) قرار گرفتند و در زمان‌های 12، 24، 36، 48 و 144 ساعت پس از اعمال تیمار بررسی شدند. نتایج نشان داد pH محیط کشت در اثر اعمال هر دو تیمار افزایش یافت. تیمارهای محرک، زنده مانی سلولی و رشد سلول ها را نسبت به شاهد کاهش دادند و این کاهش در روز ششم بعد از اعمال تیمار بیشتر بود. مقدار پراکسیدهیدروژن در تمام دوره‌های زمانی مورد ارزیابی در سلول‌های تیمار شده با متیل جاسمونات در مقایسه با سالیسیلیک اسید و شاهد بیشتر بود. مقدار پرولین و پروتئین کل و فعالیت آنزیم‌های آنتی‌اکسیدان سلول‌های تیمار شده در مقایسه با سلول‌های شاهد به طور‌ معنی‌داری افزایش یافت. حداکثر فعالیت آنزیم کاتالاز در 48 ساعت پس از تیمار سلول‌ها با سالیسیلیک اسید مشاهده شد ولی، بیشترین فعالیت آنزیم سوپراکسیددیسموتاز در 144 ساعت پس از تیمار با متیل جاسمونات بدست آمد.

کلیدواژه‌ها

عنوان مقاله [English]

Increased hydrogen peroxide and antioxidant enzymes activity in Iranian poppy cells treated with methyl jasmonate and salicylic acid

نویسندگان [English]

  • Ali Asghar Askari 1
  • naser zare 2
  • rasol asghary zakaria 3
  • Saeed khomary 4

1 MSc. Graduated student of Agricultural Biotechnology, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Associate Professor. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

3 Associate Professor. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

4 Associate Professor. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

چکیده [English]

Salicylic acid and Jasmonates are the natural occurring hormones which involved are in defense mechanisms of plant against biotic and abiotic stresses. In this research, the effect of methyl jasmonate and salicylic acid on content of hydrogen peroxide, proline and total protein and antioxidant enzymes activity were investigated. Cell suspension culture of Iranian poppy was established on liquid MS medium containing 2,4-D, kinetin and ascorbic acid. The cells treated with methyl jasmonate (100 µM) and salicylic acid (75 mg/l), and the cells were evaluated in times 12, 24, 36, 48 and 144 hours after treatment. The results indicated that medium pH increased after cells treatment with methyl jasmonate and salicylic acid. The cell growth and viability were decreased by elicitor treatments, compared to control cells and this reduction was higher on 6 days after treatment. Amount of hydrogen peroxide in the cells treated with methyl jasmonate at all assessed periods was significantly higher than those of cells treated with salicylic acid and control cells. Proline and total protein content and antioxidant enzymes activity of cells were increased significantly by methyl jasmonate and salicylic acid as compared with control cells. The highest activity of Catalase in 48 hours after treatment with salicylic acid and the highest activity of superoxide dismutase in 144 hours after treatment of cells with methyl jasmonate was obtained.

کلیدواژه‌ها [English]

  • Cloning and Expression
  • Escherichia coli
  • Rv1733c
  • Mycobacterium tuberculosis
  • Protein purifcation

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