اثرات ضد سرطانی عصارههای انگلی لیشمانیا و توکسوپلاسما بر رده سلولی سرطانی مقاوم (A2780-CP) و حساس(A2780) به سیس پلاتین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیارگروه میکروبیولوژی، د ا نشکد ه علوم زیستی، د ا نشگاه الزهرا (س،) تهران، ایران

2 استادیار گروه پرستاری،د ا نشکد ه علوم پایه، د ا نشگاه آزاد اسلامی واحد همد ا ن، همد ا ن، ایران

3 استادیار مد عوگروه زیست شناسی سلولی و مولکولی، واحد تهران شمال، د ا نشگاه آزاد اسلامی،تهران، ایران

چکیده

سرطان یکی از معضلات جامعه بشری است که تهدید کننده سلامت انسان محسوب میشود. از آنجایی که داروهای ضد سرطان دارای عوارض جانبی متعددی میباشند شناسایی فرآیندهای بیولوژیک و آنتی ژنهای تک یاختگان منجر به معرفی کاندیدهای مناسب می شود. در این مطالعه تجربی تاثیر داروی سیس پلاتین و عصاره های انگل توکسوپلاسما گوندی و لیشمانیا ماژور بر رده سلولی سرطانی مقاوم (A2780-CP) و حساس (A2780) به سیس پلاتین به صورت منفرد و توأم مورد ارزیابی قرار گرفت.جهت بررسی میزان حیات سلولهااز تست MTT وجهت بررسی میزان تکثیرسلول وشمارش سلول‌ ها از رنگ آمیزی تری پانبلواستفاده شد. داده ها توسط نرم افزار SPSS وGraphpad prism5 محاسبه گردید.میزان تکثیر سلولی در دو گروه آزمایشی نسبت به گروه کنترل با کاهش معناداری همراه بوده است. بیشترین میزان کاهش تکثیر و سلولهای مرده در دو گروه آزمایشی مربوط به گروه تیمار شده با عصارهی انگل لیشمانیا ماژور توام با داروی سیس پلاتین مشاهده گردید. عصاره های انگلی توکسوپلاسما گوندی و لیشمانیا ماژوربه دلیل دارا بودن کمپلکسی ازآنتی ژن های دفعی/ترشحی، میتوانند اثرات کشندگی قابل توجهی بر هر دو رده سلولی سرطانی دارا باشند.

کلیدواژه‌ها

عنوان مقاله [English]

Anticancer effects of parasite extracts of leishmaniasis and toxoplasma On resistant cell line (A2780-CP) and sensitive (A2780) to cisplatin

نویسندگان [English]

  • ameneh Elikaei 1
  • Hossein Vazini 2
  • Fatemeh Javani 3

1 Microbiology Department, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

2 Nursing Department, Hamedan Branch, Islamic Azad University, Hamedan, Iran

3 . Microbiology Department, TehranNorth Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Cancer is one of the problems of human society that threatens human health. Since anti-cancer drugs have multiple side effects, identification of the biological processes and the protozoan antigens leads to the introduction of suitable candidates. In this experimental study, the effect of cisplatinum and Toxoplasma gondii and Leishmania major peptic ulcers on isolated, susceptible (A2780-CP) and susceptible (A2780) and resistant strains (A2780) to Cysplatinum was evaluated individually and combined. MTT test was used to determine the cell survival and trypan blue coloring was used to determine the cell proliferation and cell count. Data were calculated using SPSS software and GraphPad prism5 software. The amount of cell proliferation in the two experimental groups was significantly lower than the control group. The highest reduction in proliferation and dead cells was observed in two experimental groups in the group treated with Leishmania major peptic ulcer extract with cisplatin. Parasitic extracts of Toxoplasma gondii and Leishmania Major can have significant lethal effects on both cancer cell lines due to their complex excretion / secretion antigens.

کلیدواژه‌ها [English]

  • Toxoplasma gondii
  • Cervical cancer
  • Cisplatinum
  • Leishmania major
  • A2780
  • A2780-CP

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