تولید کالوس از بذر بالغ سورگوم شیرین ( Sorghum bicolor L)

نوع مقاله: مقاله پژوهشی

نویسندگان

چکیده

در مطالعه حاضر با توجه به اهمیت گیاه سورگوم شیرین اندامهای مختلف گیاه شامل قطعات برگ، ریشه و بذر بالغ از گیاهان رشد یافته در شرایط کشت این ویترو در محیط کشت MS روی محیط کشت های حاوی ترکیب مختلفی از هورمونهای IBA, NAA, 2,4-D, Kinetin,  کشت گردید. در بین قطعات کشت شده تنها بخشهای تازه روئیده از بذر بالغ جوانه زده روی محیط کشت حاوی 2,4-D و Kinetin توانست پس از 1 تا 3 ماه کالوس تولید کند.

کلیدواژه‌ها

عنوان مقاله [English]

Callus production from mature seeds of Sorghum bicolor L.

نویسندگان [English]

  • Fariba Hadian
  • Ali akbar Ehsan pour
  • Abbas Almodares

چکیده [English]

In the present study, based on the importance of sorghum plant different explants including leaf, root segments and mature seed fron in vitro grown plant on MS medium were cultured on the same medium containing Kinetin, 2,4-D, IBA and NAA. Among explants only segments from germinated seeds were able to produce callus after 1-3 months.

کلیدواژه‌ها [English]

  • Callus
  • sweet sorghum
  • in vitro culture

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