بررسی اثر نیترات نقره بر ترکیبات و فعالیت آنتی اکسیدانی در کالوسهای حاصل از جدا کشت برگ گیاه اسطوخودوس(Lavandula angostifolia)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 فارق ااتحصیل از مقطع کارشناسی ارشد رشته فیزیوژی گیاهی،دانشکده علوم زیسی،دانشگاه الزهرا، ایران

2 مدیر گروه فیزیولوژی گیاهی،دانشکده علوم زیستی ،دانشگاه الزهرا،ایران

3 استادیار گروه فیزیولوژی گیاهی، دانشکده علوم زیستی، دانشگاه الزهرا، ایران

چکیده

اسطوخودوس با نام علمی Lavandula angustifolia گیاهی است متعلق به خانواده نعنا عیان ، تمام اندام های این گیاه غنی از ترکیبات ثانویه با خاصیت آنتی اکسیدانی بالا است و می تواند منبع مناسبی برای تهیه آنتی اکسیدانها در شرایط کشت بافت باشد. در این بررسی با هدف افزایش متابولیت های ثانویه با خاصیت آنتی اکسیدانی از الیسیتور غیر زیستی نیترات نقره استفاده شد. میزان 1،2،4و6mg/l نیترات نقره به کالوسهای یک ماهه رشد کرده در محیطMS دارای هورمون های نفتالن استیک اسید (NAA ) mg/l 2وبنزیل آمینوپورین(BAP)mg/l 4 اضافه شد و پس از یک ماه کالوسها برداشت و آنالیزها انجام شد. نتایج نشان داد با افزایش غلظت نیترات نقره در محیط کشت،میزان رشد کالوسها افزایش یافت و افزایش میزان نیترات نقره تا غلظت mg/l 4سبب افزایش میزان رزمارینیک اسید ،ترکیبات فنلی و فلاونوئید و همچنین فعالیت آنتی اکسیدانی شد،اما در غلظت های بالاتر از mg/l4 اثرات سمی یونهای نقره آشکار شد و باعث کاهش میزان رزمارینیک اسید ،ترکیبات فنلی و همچنین فعالیت آنتی اکسیدانی شد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation effect of silver nitrate on antioxidant activity and antioxidant compounds in calli frome leaves of lavandula angostifolia.

نویسندگان [English]

  • Firuze Alirezayi 1
  • Khadijah Kiarostami 2
  • Monia Hussein Zadeh Namin 3

1 Graduate from the Master's degree in Plant Physiology, Faculty of Science, Zazi University, Alzahra University, Iran

2 Director of the Department of Plant Physiology, Faculty of Life Sciences, Al-Zahra University, Iran

3 Assistant Professor of Plant Physiology, Faculty of Life Sciences, Alzahra University, Iran

چکیده [English]

Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants secondry methabolites with high antioxidant properties ,silver nitrate as abiotic elicitor used in tissue culture of Lavandula angutifolia. Concentrations of 1, 2, 4 and 6 mg/l of silver nitrate as abiotic elicitor added to the one-month calli from leaf explant grown in MS medium with , Naphthaleneacetic acid(NAA)2mg/l, Benzyl amino purine (BAP) 4mg/l.After a month calli were harvested and the analyses were performed.The results showed that with increasing concentration of silver nitrate in the culture medium, growth of calli was increased and increasing the amount of silver nitrate concentration up to 4mg / l, increased Rosmarinic acid, flavonoids ,phenolic compounds and antioxidant activity .But in higher concentration of 4 mg / l the toxic effects of silver ions revealed and reduced the amount of Rosmarinic acid,phenolic compounds and antioxidant activity.

کلیدواژه‌ها [English]

  • Lavendula angostifolia
  • Rosmarinic acid
  • phenolic compounds
  • flavonoids
  • anti� oxidant activity

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