حفاظت کلسیم‌کلرید از گیاه پریوش Catharanthus roseus تحت تنش شوری با القاء سیستم دفاع آنتی‌اکسیداتیو و افزایش رنگیزه‌های فتوسنتزی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی گیاهی، گروه زیست شناسی ، دانشگاه اراک، اراک ، ایران

2 استادیار فیزیولوژی گیاهی،گروه زیست شناسی ، دانشگاه اراک ، اراک ،ایران

3 دانشجوی کارشناسی ارشد ، فیزیولوژی گیاهی ، گروه زیست شناسی ، دانشگاه اراک ، اراک ، ایران

چکیده

این مطالعه اثرات کلسیم‌ کلرید را به عنوان یک عامل تعدیل‌ کننده تنش شوری بر گیاه پریوش بررسی می‌کند. گیاهان 49 روزه با غلظت‌های مختلف کلرید سدیم (0، 35، 70 و 100 میلی‌مولار) به تنهایی و توام با غلظت‌های مختلف کلرید کلسیم (0، 5 و 10 میلی‌مولار) به مدت 21 روز تیمار شدند. سپس درصد بازدارندگی رادیکال دی‌فنیل-2- پیکریل‌هیدرازیل (I%)، فعالیت آنزیم‌های آنتی‌اکسیدان، سطح و تعداد برگ و مقدار رنگیزه‌های فتوسنتزی اندازه‌گیری شدند. نتایج کاهش معنی‌دار مقدار رنگیزه‌ها، سطح و تعداد برگ را تحت تنش شوری نشان داد. افزودن کلسیم‌ کلرید به گیاهان تحت تنش شوری، مقادیر رنگیزه‌ها، سطح برگ، فعالیت آنزیم‌های آنتی‌اکسیدان و I% را در مقایسه با گیاهان رشد یافته تحت تنش شوری به تنهایی افزایش داد. بیشترین افزایش در تیمارهای ترکیبی (100 میلی‌مولار کلرید سدیم + 5 میلی‌مولار کلرید کلسیم) مشاهده شد. نتایج اثرات مفید تغذیه کلسیم بر رشد گیاه پریوش تحت تنش شوری را مطرح می‌کند.

کلیدواژه‌ها

عنوان مقاله [English]

Protection of CaCl2 of Catharanthus roseus under salinity stress by inducing antioxidative defence system and increase of photosynthetic pigments

نویسندگان [English]

  • Mehri Assgari 1
  • Fariba Amini 2
  • Soheila Shokri 3

1 Assistant Professor of Plant Physiology, Department of Biology, Arak University, Arak, Iran

2 Assistant Professor of Plant Physiology, Department of Biology, Arak University, Arak, Iran

3 MSc Student, Plant Physiology, Department of Biology, Arak University, Arak, Iran

چکیده [English]

This study evaluated the effects of calcium chloride as alleviator agent in salinity stress on Catharanthus roseus. 49- day- old plants were treated with different concentrations of NaCl (0, 35, 70 and 100 mM) alone and in combination of various CaCl2 concentration (0, 5 and 10 Mm) for 21 days. Then, percent of Inhibition of 1,1-diphenyl-2- picrylhydrazyl radical (I%), activity of antioxidant enzymes, contents of photosynthetic pigments, leaf number and area were measured. The results indicated that pigments contents, leaf area and number were significantly decreased by salinity. Addition of CaCl2 to salinity-stressed plants increased of pigments contents, leaf area, the activitiy of antioxidant enzymes and I% in compared to plants grown with NaCl alone. The maximum enhancement was observed with the combined treatments (100mM NaCl + 5mM CaCl2). The results are discussed in terms of the beneficial effects of calcium supply on the growth of vinca under saline conditions.

کلیدواژه‌ها [English]

  • Catalase
  • diphenyl�2�picrylhydrazyl radical (DPPH)
  • Growth parameters
  • guaiacol peroxidase
  • superoxide dismutase

 

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