بررسی تاثیر شدت نور و pH بر میزان رشد، محتوای پروتئین و چربی در Spirulina platensis

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران

2 استادیار، گروه زیست‌شناسی دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران

3 استاد گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران.

چکیده

سیانوباکتریArthrospira (Spirulina) platensis یک جلبک سبز آبی رشته ای است که دارای ارزش غذایی و دارویی بسیاری برای انسان و جانوران می باشد. دراین مطالعه تاثیر شدت نور و pH بر میزان تولید پروتئین، چربی، کلروفیل و کارتنوئید ها در       Spirulina platensis مورد بررسی قرار گرفته است. نمونه خالص اسپیرولینا در محیط کشت BG-11 مایع تحت تاثیر3 شدت نوری  (Lux 1500 ,1000(شاهد ) , 500 ) وپنج تیمار pH شامل (1/9 ,1/8 ,1/7 (شاهد) ,1/6, 1/5 ) رشد داده شد و در شرایط بهینه به مدت 25 روز تیماردهی گردید. نتایج نشان داد افزایش شدت نور تاثیر معنی‌داری در میزان تولید چربی در این سیانوباکتری دارد ولی در میزان تولید پروتئین و رنگدانه های فتوسنتزی تاثیر معنی داری مشاهده نگردید. همچنین نتایج حاصل از بررسی اثرگذاری تغییرات  pH درSpirulina platensis نشان داد که pH قلیایی در تولید چربی و پروتئین از pH خنثی مناسب تر بوده و بطور معنی‌داری سبب افزایش میزان این ترکیبات در سیانوباکتری اسپیرولینا می‌شود.  

کلیدواژه‌ها

عنوان مقاله [English]

Effects of light intensity and pH on the growth rate, lipid and protein content in Spirulina platensis

نویسندگان [English]

  • masoumeh Helmi-Seresht 1
  • sara Saadatmand 2
  • ramezanali Khavari-Nejad 3

چکیده [English]

Cyanobacterium Arthrospira (Spirulina) platensis  is a filamentous blue-green algae which has many nutritional and medicinal values for humans and animals. The present study investigated the effect of light intensity and pH on the productions of protein, fat, chlorophyll a and carotenoids in Spirulina platensis. Pure sample of Spirulina was grown in BG-11 liquid culture medium under the influence of three optical intensities including 1500, 1000 (control), and 500 Lux and five pH treatments including 5.1, 6.1, 7.1 (control), 8.1, 9.1 and fintensity had a statistically significant impact on the amount of fat production in the cyanobacterium. However, no significant difference was observed in the amounts of the protein and photosynthetic pigments productions. The results of investigating the influence of pH changes in Spirulina platensis indicated that alkaline pH was more suitable in the productions of fat and protein than neutral pH and resulted in a significant increase in the amount of these compounds in cyanobacterium Spirulina.  

کلیدواژه‌ها [English]

  • Spirulina Platensis
  • pH
  • light intensity
  • Protein
  • lipid.  
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